| 货号 | 9197T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human, Mouse, Rat, Monkey, D. melanogaster |
| 应用 | WB,IP,IHC,IF,F,CHIP |
| 目标/特异性 | CREB (48H2) Rabbit mAb detects endogenous levels of total CREB-1 protein. The antibody does not cross-react with other ATF/CREB family members. |
| 使用方法 | Western Blotting (1:1000) Immunoprecipitation (1:250) Immunohistochemistry (Paraffin) (1:6400) Immunohistochemistry (Frozen) (1:6400) Immunofluorescence (Frozen) (1:800) Immunofluorescence (Immunocytochemistry) (1:800) Flow Cytometry (1:400) Chromatin IP (1:50) Chromatin IP-seq (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | CREB is a bZIP transcription factor that activates target genes through cAMP response elements. CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth, and neuronal differentiation in certain neuronal populations (1-3). Additionally, CREB signaling is involved in learning and memory in several organisms (4-6). CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. CREB is activated by phosphorylation at Ser133 by various signaling pathways including Erk, Ca2+, and stress signaling. Some of the kinases involved in phosphorylating CREB at Ser133 are p90RSK, MSK, CaMKIV, and MAPKAPK-2 (7-9). |
| 存放说明 | -20C |
| 计算分子量 | 43 |
| 参考文献 | 1 . Lonze, B.E. et al. (2002) Neuron 34, 371-85. 2 . Lee, M.M. et al. (1999) J Neurosci Res 55, 702-12. 3 . Redmond, L. et al. (2002) Neuron 34, 999-1010. 4 . Dash, P.K. et al. (1990) Nature 345, 718-21. 5 . Yin, J.C. et al. (1994) Cell 79, 49-58. 6 . Guzowski, J.F. and McGaugh, J.L. (1997) Proc Natl Acad Sci USA 94, 2693-8. 7 . Xing, J. et al. (1998) Mol Cell Biol 18, 1946-55. 8 . Ribar, T.J. et al. (2000) J Neurosci 20, RC107. 9 . Tan, Y. et al. (1996) EMBO J 15, 4629-42. |
Immunohistochemical analysis of frozen H1650 xenograft, showing nuclear localization using CREB (48H2) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded mouse brain, using CREB (48H2) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using CREB (48H2) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human Non-Hodgkins lymphoma, using CREB (48H2) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human astrocytoma, using CREB (48H2) Rabbit mAb. | |
Western Blot analysis of extracts from SK-N-MC, COS, NIH/3T3, C6 and Drosophila S2 cells, using CREB (48H2) Rabbit mAb. | |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 293 cells, treated with Forskolin #3828 (30 μM) for 1h and either 10 μl of CREB (48H2) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729, using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human ALS2 exon 1 primers, SimpleChIP® Human NR4A3 Promoter Primers #4829, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. | |
Confocal immunofluorescent analysis of mouse cerebellum labeled with CREB (48H2) Rabbit mAb (red) and Neurofilament-L (DA2) Mouse mAb #2835 (green). Blue pseudocolor =DRAQ5® #4084 (fluorescent DNA dye). | |
Confocal immunofluorescent analysis of SK-N-MC cells showing nuclear stain with CREB (48H2) Rabbit mAb (A, red) compared to an isotype control (B). Blue pseudocolor =DRAQ5® (fluorescent DNA dye). | |
Flow cytometric analysis of SK-N-MC cells using CREB (48H2) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red). | |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 293 cells treated with Forskolin #3828 (30 μM) for 1h and 10 μl of CREB (48H2) Rabbit mAb, using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. DNA Libraries were prepared from 5 ng enriched ChIP DNA using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina®, and sequenced on the Illumina NextSeq. The figure shows binding across NR4A3, a known target gene of CREB (see additional figure containing ChIP-qPCR data). For additional ChIP-seq tracks, please download the product data sheet. |
