| 货号 | 86298T |
| 同种亚型 | Mouse IgG2b |
| 反应种属 | H M R Hm Mk |
| 来源宿主 | Mouse IgG2b |
| 应用 | W IHC-P IF-IC |
| 目标/特异性 | β-Tubulin (D3U1W) Mouse mAb recognizes endogenous levels of total β-tubulin protein. |
| 使用方法 | WB(1:1000) IHC-P (1:800) IF-IC (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1). |
| 运输条件 | 0.75 |
| 存放说明 | -20C |
| 计算分子量 | 55 |
| 参考文献 | 1 . Westermann, S. and Weber, K. (2003) Nat Rev Mol Cell Biol 4, 938-47. |
Confocal immunofluorescent analysis of C2C12 cells using β-Tubulin (D3U1W) Mouse mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using β-Tubulin (D3U1W) Mouse mAb. | |
Immunohistochemical analysis of paraffin-embedded human large cell carcinoma of the lung using β-Tubulin (D3U1W) Mouse mAb. | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using β-Tubulin (D3U1W) Mouse mAb. | |
Immunohistochemical analysis of paraffin-embedded human endometrioid adenocarcinoma using β-Tubulin (D3U1W) Mouse mAb. |
