| 货号 | 15126T |
| 同种亚型 | Mouse IgG1 |
| 反应种属 | H M |
| 来源宿主 | Mouse IgG1 |
| 应用 | W IHC-P IF-F |
| 目标/特异性 | β-Amyloid (D3D2N) Mouse mAb recognizes endogenous levels of total β-amyloid peptide (Aβ). This product detects transgenically expressed human APP in mouse models. |
| 使用方法 | WB(1:1000) IHC-P (1:800) IF-F (1:200) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Amyloid β (Aβ) precursor protein (APP) is a 100-140 kDa transmembrane glycoprotein that exists as several isoforms (1). The amino acid sequence of APP contains the amyloid domain, which can be released by a two-step proteolytic cleavage (1). The extracellular deposition and accumulation of the released Aβ fragments form the main components of amyloid plaques in Alzheimers disease (1). APP can be phosphorylated at several sites, which may affect the proteolytic processing and secretion of this protein (2-5). Phosphorylation at Thr668 (a position corresponding to the APP695 isoform) by cyclin-dependent kinase is cell-cycle dependent and peaks during G2/M phase (4). APP phosphorylated at Thr668 exists in adult rat brain and correlates with cultured neuronal differentiation (5,6). |
| 运输条件 | 0.75 |
| 存放说明 | -20C |
| 计算分子量 | 5 |
| 参考文献 | 1 . Selkoe, D.J. (1996) J Biol Chem 271, 18295-8. 2 . Caporaso, G.L. et al. (1992) Proc Natl Acad Sci USA 89, 3055-9. 3 . Hung, A.Y. and Selkoe, D.J. (1994) EMBO J 13, 534-42. 4 . Suzuki, T. et al. (1994) EMBO J 13, 1114-22. 5 . Ando, K. et al. (1999) J Neurosci 19, 4421-7. 6 . Iijima, K. et al. (2000) J Neurochem 75, 1085-91. |
Confocal immunofluorescent analysis of brain sections from 13-month old wild-type (left) and Tg2576 (center) mice using β-Amyloid (D3D2N) Mouse mAb (green) and β3-Tubulin (D71G9) XP® Rabbit mAb #5568 (red). A detailed view of a cortical β-amyloid plaque is shown on the right. Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Western blot analysis of human Aβ-37, Aβ-40, and Aβ-42 peptides using β-Amyloid (D3D2N) Mouse mAb. | |
Immunohistochemical analysis of paraffin-embedded human Alzheimers brain using β-Amyloid (D3D2N) Mouse mAb. |
