β-Amyloid (1-42 Specific) (D9A3A) Rabbit mAb【科瑞奥博】

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β-Amyloid (1-42 Specific) (D9A3A) Rabbit mAb

货号： 14974T 基本售价： 1350.0 元规格： -

产品信息

概述
货号	14974T
同种亚型	Rabbit IgG
反应种属	H M
来源宿主	Rabbit IgG
应用	W IF-F
目标/特异性	β-Amyloid (1-42 Specific) (D9A3A) Rabbit mAb recognizes endogenous levels of total Aβ-42 peptide. This antibody may cross-react with Aβ-43 peptide. This product detects transgenically expressed human APP in mouse models.
使用方法	WB(1:1000) IF-F (1:1600)

性能
供应商	CST
灵敏度	Endogenous
背景	Amyloid β (Aβ) precursor protein (APP) is a 100-140 kDa transmembrane glycoprotein that exists as several isoforms (1). The amino acid sequence of APP contains the amyloid domain, which can be released by a two-step proteolytic cleavage (1). The extracellular deposition and accumulation of the released Aβ fragments form the main components of amyloid plaques in Alzheimers disease (1). APP can be phosphorylated at several sites, which may affect the proteolytic processing and secretion of this protein (2-5). Phosphorylation at Thr668 (a position corresponding to the APP695 isoform) by cyclin-dependent kinase is cell-cycle dependent and peaks during G2/M phase (4). APP phosphorylated at Thr668 exists in adult rat brain and correlates with cultured neuronal differentiation (5,6).
运输条件	0.75
存放说明	-20C
计算分子量	4
参考文献	1 . Selkoe, D.J. (1996) J Biol Chem 271, 18295-8. 2 . Caporaso, G.L. et al. (1992) Proc Natl Acad Sci USA 89, 3055-9. 3 . Hung, A.Y. and Selkoe, D.J. (1994) EMBO J 13, 534-42. 4 . Suzuki, T. et al. (1994) EMBO J 13, 1114-22. 5 . Ando, K. et al. (1999) J Neurosci 19, 4421-7. 6 . Iijima, K. et al. (2000) J Neurochem 75, 1085-91.

参考图片

Confocal immunofluorescent analysis of wild-type (left) and Tg2576 mouse model of Alzheimers brain (right) using β-Amyloid (1-42 Specific) (D9A3A) Rabbit mAb (green) and β3-Tubulin (TU-20) Mouse mAb #4466 (red). Blue pseudocolor = DRAQ5^® #4084 (fluorescent DNA dye).

Western blot analysis of human Aβ-37, Aβ-38, Aβ-39, Aβ-40, and Aβ-42 peptides (2.5 ng) using β-Amyloid (1-42 Specific) (D9A3A) Rabbit mAb (upper) and β-Amyloid (D54D2) XP^® Rabbit mAb #8243 (lower).

Western blot analysis of brain extracts from 13-month old wild-type and Tg2576 mouse model of Alzheimers brain using β-Amyloid (1-42 Specific) (D9A3A) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).