| 货号 | 3274T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human,Mouse,Rat,Monkey, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP |
| 目标/特异性 | GβL (86B8) Rabbit mAb detects endogenous levels of total GβL protein. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Cell growth is a fundamental biological process whereby cells accumulate mass and increase in size. The mammalian Target of Rapamycin (mTOR) pathway regulates growth by coordinating energy and nutrient signals with growth factor-derived signals (1). mTOR is a large protein kinase with two different complexes. One complex contains mTOR, GβL, and raptor, which is a target of rapamycin. The other complex, insensitive to rapamycin, includes mTOR, GβL, and rictor (1). GβL associates with the kinase domain of mTOR and stimulates mTOR kinase activity (2). A reduction in GβL expression has been shown to decrease in vivo phosphorylation of S6K1 (2). |
| 存放说明 | -20C |
| 计算分子量 | 37 |
| 参考文献 | 1 . Sarbassov, D.D. et al. (2004) Curr. Biol. 14, 1296-1302. 2 . Kim, D.H. et al. (2003) Mol. Cell 11, 895-904. |
Western blot analysis of extracts from GβL wild-type (+/+) and knockout (-/-) mouse embryonic fibroblast cells using GβL (86B8) Rabbit mAb. (provided by David Sabatini Lab at the Whitehead Institute for Biomedical Research). Western blot检测分析GβL 野生型 (+/+) 和敲除型(-/-) 鼠胚胎成纤维细胞提取物，使用的抗体是GβL (86B8) Rabbit mAb兔单抗。（由David Sabatini Lab，the Whitehead Institute for Biomedical Research提供）。 | |
Western blot analysis of extracts from various cell lines, using GβL (86B8) Rabbit mAb. Western blot 检测分析各种细胞提取物，使用抗体是 GβL (86B8) Rabbit mAb兔单抗。 |
