| 货号 | 6978T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB |
| 目标/特异性 | TACE (D22H4) Rabbit mAb recognizes endogenous levels of total TACE protein. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | TACE (TNF-α converting enzyme), also known as ADAM17, is a transmembrane metalloprotease that plays a key role in the cleavage of a number cell surface molecules in a process known as “shedding". TACE is abundantly expressed in many adult tissues, but in fetal development expression is differentially regulated (1). An important substrate of TACE is pro-TNF-α (1). Increased expression of TACE is associated with several pathological conditions including osteoarthritis and rheumatoid arthritis, where the pro-inflammatory effects of increased TNF-α contribute to disease pathogenesis (2,3). Regulation of other important molecules by TACE such as EGFR and Notch has recently been documented. TACE is responsible for the shedding of EGFR ligands such as amphiregulin and TNF-α. Some tumors have hyperactivated EGFR due to upregulated TNF-α production and upregulated TACE, making TACE a potential target for drug development (4). TACE activates Notch in a ligand-independent manner and has been shown to play a role in the development of the Drosophila nervous system (5). TACE has also been proposed to act as α-secretase for amyloid precursor protein (APP) (6), and to be involved in the renewal and proliferation of neural stem cells (7). |
| 存放说明 | -20C |
| 计算分子量 | 135 |
| 参考文献 | 1 . Black, R.A. et al. (1997) Nature 385, 729-33. 2 . Amin, A.R. (1999) Osteoarthritis Cartilage 7, 392-4. 3 . Patel, I.R. et al. (1998) J Immunol 160, 4570-9. 4 . Kenny, P.A. (2007) Differentiation 75, 800-8. 5 . Delwig, A. and Rand, M.D. (2008) Cell Mol Life Sci 65, 2232-43. 6 . Deuss, M. et al. (2008) Curr Alzheimer Res 5, 187-201. |
Western blot analysis of extracts from Raji and Jurkat cells, untreated or treated with peptide N-glycosidase F (PNGase F), using TACE (D22H4) Rabbit mAb.未处理或经过肽N-糖苷酶（PNGase F）处理的Raji和Jurkat细胞，获得提取物，使用TACE (D22H4) 兔单抗进行western blot实验。 |
