| 货号 | 6946T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP |
| 目标/特异性 | PINK1 (D8G3) Rabbit mAb recognizes endogenous levels of total PINK1 protein. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | PTEN induced putative kinase 1, PINK1, is a mitochondrial serine/threonine kinase involved in the normal function and integrity of mitochondria, as well as in reduction of cytochrome c release from mitochondria (1-3). PINK1 phosphorylates Parkin and promotes its translocation to mitochondria (2). Research studies have shown that mutations in PINK1 are linked to autosomal recessive early onset Parkinson’s disease, and are associated with loss of protective function, mitochondrial dysfunction, aggregation of α-synuclein, as well as proteasome dysfunction (1,3). |
| 存放说明 | -20C |
| 计算分子量 | 60, 50 |
| 参考文献 | 1 . Liu, W. et al. (2009) PLoS One 4, e4597. 2 . Kim, Y. et al. (2008) Biochem Biophys Res Commun 377, 975-80. 3 . Petit, A. et al. (2005) J Biol Chem 280, 34025-32. |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a cDNA construct expressing full-length human PINK1 (hPINK1, +) using PINK1 (D8G3) Rabbit mAb. | |
Western blot analysis of extracts from HeLa cells, untreated (-) or treated with CCCP (10 μM, 24 hr; +), using PINK1 (D8G3) Rabbit mAb. |
