ASC (D2W8U) Rabbit mAb (Mouse Specific)【科瑞奥博】

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ASC (D2W8U) Rabbit mAb (Mouse Specific)

货号： 67824T 基本售价： 1350.0 元规格： -

产品信息

概述
货号	67824T
同种亚型	Rabbit IgG
反应种属	Mouse
应用	WB,IP,IF,F
目标/特异性	ASC/TMS1 (D2W8U) Rabbit mAb (Mouse Specific) recognizes endogenous levels of total ASC/TMS1 protein.
使用方法	Western Blotting (1:1000) Immunoprecipitation (1:100) Immunofluorescence (Immunocytochemistry) (1:800) Flow Cytometry (1:800)

性能
供应商	CST
灵敏度	Endogenous
背景	TMS1 (target of methylation-induced silencing)/ASC (apoptosis-associated speck-like protein containing a CARD), also referred to as PYCARD and CARD5, is a 22-kDa pro-apoptotic protein containing an N-terminal pyrin domain (PYD) and a C-terminal caspase recruitment domain (CARD) (1-2). The ASC/TMS1 gene was originally found to be aberrantly methylated and silenced in breast cancer cells (2), and has since been found to be silenced in a number of other cancers, including ovarian cancer (3), glioblastoma (4), melanoma (5), gastric cancer (6), lung cancer (7), and prostate cancer (8). Expression of ASC/TMS1 can be induced by pro-apoptotic/inflammatory stimuli (9). During apoptosis ASC/TMS1 is re-distributed from the cytosol to the mitochondria and associates with mitochondrial Bax to trigger cytochrome c release and subsequent apoptosis (10). ASC/TMS1 has also been found to be a critical component of inflammatory signaling where it associates with and activates caspase-1 in response to pro-inflammatory signals (11).
存放说明	-20C
计算分子量	22
参考文献	1 . Masumoto, J. et al. (1999) J Biol Chem 274, 33835-8. 2 . Conway, K.E. et al. (2000) Cancer Res 60, 6236-42. 3 . Terasawa, K. et al. (2004) Clin Cancer Res 10, 2000-6. 4 . Stone, A.R. et al. (2004) Am J Pathol 165, 1151-61. 5 . Guan, X. et al. (2003) Int J Cancer 107, 202-8. 6 . Moriai, R. et al. (2002) Anticancer Res 22, 4163-8. 7 . Virmani, A. et al. (2003) Int J Cancer 106, 198-204. 8 . Das, P.M. et al. (2006) Mol Cancer 5, 28. 9 . Strong, R. et al. (1991) Brain Res 542, 23-8. 10 . Ohtsuka, T. et al. (2004) Nat Cell Biol 6, 121-8. 11 . Srinivasula, S.M. et al. (2002) J Biol Chem 277, 21119-22.

参考图片

Western blot analysis of extracts from J774A.1 and Raw 264.7 cells using ASC/TMS1 (D2W8U) Rabbit mAb (Mouse Specific) (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

Immunoprecipitation of ASC/TMS1 from J774A.1 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP^® Isotype Control #3900, and lane 3 is ASC (D2W8U) Rabbit mAb (Mouse Specific). Western blot analysis was performed using ASC/TMS1 (D2W8U) Rabbit mAb (Mouse Specific).

Confocal immunofluorescent analysis of mouse primary bone marrow-derived macrophages (BMDMs) either untreated (upper left) or treated with LPS (50 ng/ml, 4 hr, middle) or LPS followed by ATP (5 mM, 45 min, upper right), and J774A.1 (lower left) or Raw 264.7 (lower right) cells, using ASC/TMS1 (D2W8U) Rabbit mAb (Mouse Specific) (green). Blue pseudocolor = DRAQ5^® #4084 (fluorescent DNA dye). Note the translocation of ASC to inflammasomes following stimulation with LPS and ATP (white arrows).

Flow cytometric analysis of Raw264.7 cells (blue) and J774A.1 cells (green) using ASC/TMS1 (D2W8U) Rabbit mAb (Mouse Specific) (solid lines) or a concentration-matched Rabbit (DA1E) mAb IgG XP^®Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab)₂Fragment (Alexa Fluor^®488 Conjugate) #4412 was used as a secondary antibody.