| 货号 | 2869T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 目标/特异性 | Syntaxin 6 (C34B2) Rabbit mAb detects endogenous levels of total syntaxin 6 protein. |
| 使用方法 | WB(1:2000) IP (1:50) IF-IC (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Syntaxin 6 is a ubiquitously expressed S25C family member of the SNARE proteins (1,2). The protein has an amino-terminal H1 domain followed by an H2 SNARE domain and a carboxy-terminal membrane anchor (3). Syntaxin 6 protein is localized to the trans-Golgi and within endosomes and regulates membrane trafficking by partnering with a variety of other SNARE proteins (3-5). Depending on cell type and SNARE parter, syntaxin 6 is involved in the regulation of GLUT4 trafficking, neutrophil exocytosis and granule secretion (6-10). |
| 存放说明 | -20C |
| 计算分子量 | 32 |
| 参考文献 | 1 . Bock, J.B. et al. (2001) Nature 409, 839-41. 2 . Bock, J.B. et al. (1996) J Biol Chem 271, 17961-5. 3 . Wendler, F. and Tooze, S. (2001) Traffic 2, 606-11. 4 . Bock, J.B. et al. (1997) Mol Biol Cell 8, 1261-71. 5 . Mallard, F. et al. (2002) J Cell Biol 156, 653-64. 6 . Perera, H.K. et al. (2003) Mol Biol Cell 14, 2946-58. 7 . Shewan, A.M. et al. (2003) Mol Biol Cell 14, 973-86. 8 . Martín-Martín, B. et al. (2000) Blood 96, 2574-83. 9 . Wendler, F. et al. (2001) Mol Biol Cell 12, 1699-709. 10 . Kuliawat, R. et al. (2004) Mol Biol Cell 15, 1690-701. |
Western blot analysis of extracts from various cell lines using Syntaxin 6 (C34B2) Rabbit mAb. 对多种细胞系使用Syntaxin 6 (C34B2) Rabbit mAb兔单抗进行Western blot。 | |
Confocal immunofluorescent analysis of MCF-7 cells using Syntaxin 6 (C34B2) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye). 对MCF-7细胞使用Syntaxin 6 (C34B2) Rabbit mAb兔单抗（绿色）进行共聚焦免疫荧光分析。肌动蛋白用DY-554 phalloidin（红色）标记。蓝色伪彩=DRAQ5®(荧光DNA染料)。 |
