| 货号 | 2867T |
| 同种亚型 | Rabbit |
| 反应种属 | Human,Mouse,Rat,Monkey, |
| 来源宿主 | Rabbit |
| 应用 | WB |
| 目标/特异性 | Hexokinase II (C64G5) Rabbit mAb detects endogenous levels of total hexokinase II protein. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Hexokinase catalyzes the conversion of glucose to glucose-6-phosphate, the first step in glycolysis. Four distinct mammalian hexokinase isoforms, designated as hexokinase I, II, III and IV (glucokinase), have been identified. Hexokinases I, II and III are associated with the outer mitochondrial membrane and are critical for maintaining an elevated rate of aerobic glycolysis in cancer cells (Warburg Effect) (1) in order to compensate for the increased energy demands associated with rapid cell growth and proliferation (2,3). |
| 存放说明 | -20C |
| 计算分子量 | 102 |
| 参考文献 | 1 . WARBURG, O. (1956) Science 123, 309-14. 2 . Semenza, G.L. (2000) Crit Rev Biochem Mol Biol 35, 71-103. 3 . Smith, T.A. (2000) Br J Biomed Sci 57, 170-8. |
Western blot analysis of various cell extracts using Hexokinase II (C64G5) Rabbit mAb (upper) and β-Actin (13E5) Rabbit mAb #4970 (lower). |
