| 货号 | 9129T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human,Mouse,Rat, |
| 来源宿主 | Rabbit IgG |
| 应用 | F, IF-IC |
| 目标/特异性 | Ki-67 (D3B5) Rabbit mAb recognizes endogenous levels of total Ki-67 protein. |
| 使用方法 | F(1:400) IF-IC (1:400) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Ki-67, named after the location where it was discovered (Kiel University, Germany), is a nuclear nonhistone protein (1) that is universally expressed among proliferating cells and absent in quiescent cells (2). Ki-67 detects proliferating cells in G1, S, G2, and mitosis, but not in the G0 resting phase. Research studies have shown that high levels of Ki-67 are associated with poorer breast cancer survival (3). Research studies have explored the use of Ki-67, along with other markers, as potential prognostic or predictive markers in breast cancer and other malignant diseases (4).Ki-67，以其发现地命名（Kiel大学，德国），作为一个细胞核非组蛋白（1）通常在增殖的细胞中表达并且在休眠细胞中不表达（2）。Ki-67会让增殖细胞停滞在G1，S，G2和有丝分裂期，但是不会停滞在G0期。研究显示高表达的Ki-67与低乳腺癌存活率相关（3）。研究已经将Ki-67与其他的标记，作为乳腺癌和其他恶性疾病中的潜在的预后和预测标记（4）。 |
| 存放说明 | -20C |
| 计算分子量 | 359 |
| 参考文献 | 1 . Gerdes, J. et al. (1983) Int J Cancer 31, 13-20. 2 . Weigel, M.T. and Dowsett, M. (2010) Endocr Relat Cancer 17, R245-62. 3 . Jones, R.L. et al. (2009) Breast Cancer Res Treat 116, 53-68. 4 . Yerushalmi, R. et al. (2010) Lancet Oncol 11, 174-83. |
Flow cytometric analysis of Jurkat cells using Ki-67 (D3B5) Rabbit mAb and Propidium Iodide (PI)/RNase Staining Solution #4087. Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.使用Ki-67 (D3B5)Rabbit mAb和碘化丙啶/RNase固定液#4087对Jurkat细胞进行流式细胞分析。Anti-rabbit IgG (H+L), F(ab)2 Fragment (偶联Alexa Fluor® 488) #4412作为二抗。 | |
Confocal immunofluorescent analysis of HeLa cells using Ki-67 (D3B5) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).使用Ki-67 (D3B5)Rabbit mAb（绿色）对HeLa细胞进行流式细胞分析。肌动蛋白丝使用DY-554鬼笔环肽标记（红色）。 |
