| 货号 | 2938T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human,Mouse,Rat,Monkey, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP |
| 目标/特异性 | Akt1 (C73H10) Rabbit mAb detects endogenous levels of total Akt1 protein. This antibody does not cross-react with Akt2 or Akt3. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis (1-3). This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase (2,3). Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 (4) and by phosphorylation within the carboxy terminus at Ser473. The previously elusive PDK2 responsible for phosphorylation of Akt at Ser473 has been identified as mammalian target of rapamycin (mTOR) in a rapamycin-insensitive complex with rictor and Sin1 (5,6). Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including Bad (7), forkhead transcription factors (8), c-Raf (9), and caspase-9. PTEN phosphatase is a major negative regulator of the PI3 kinase/Akt signaling pathway (10). LY294002 is a specific PI3 kinase inhibitor (11). Another essential Akt function is the regulation of glycogen synthesis through phosphorylation and inactivation of GSK-3α and β (12,13). Akt may also play a role in insulin stimulation of glucose transport (12). In addition to its role in survival and glycogen synthesis, Akt is involved in cell cycle regulation by preventing GSK-3β-mediated phosphorylation and degradation of cyclin D1 (14) and by negatively regulating the cyclin dependent kinase inhibitors p27 Kip (15) and p21 Waf1/CIP1 (16). Akt also plays a critical role in cell growth by directly phosphorylating mTOR in a rapamycin-sensitive complex containing raptor (17). More importantly, Akt phosphorylates and inactivates tuberin (TSC2), an inhibitor of mTOR within the mTOR-raptor complex (18,19). |
| 存放说明 | -20C |
| 计算分子量 | 60 |
| 参考文献 | 1 . Franke, T.F. et al. (1997) Cell 88, 435-7. 2 . Burgering, B.M. and Coffer, P.J. (1995) Nature 376, 599-602. 3 . Franke, T.F. et al. (1995) Cell 81, 727-36. 4 . Alessi, D.R. et al. (1996) EMBO J 15, 6541-51. 5 . Sarbassov, D.D. et al. (2005) Science 307, 1098-101. 6 . Jacinto, E. et al. (2006) Cell 127, 125-37. 7 . Cardone, M.H. et al. (1998) Science 282, 1318-21. 8 . Brunet, A. et al. (1999) Cell 96, 857-68. 9 . Zimmermann, S. and Moelling, K. (1999) Science 286, 1741-4. 10 . Cantley, L.C. and Neel, B.G. (1999) Proc Natl Acad Sci USA 96, 4240-5. 11 . Vlahos, C.J. et al. (1994) J Biol Chem 269, 5241-8. 12 . Hajduch, E. et al. (2001) FEBS Lett 492, 199-203. 13 . Cross, D.A. et al. (1995) Nature 378, 785-9. 14 . Diehl, J.A. et al. (1998) Genes Dev 12, 3499-511. 15 . Gesbert, F. et al. (2000) J Biol Chem 275, 39223-30. 16 . Zhou, B.P. et al. (2001) Nat Cell Biol 3, 245-52. 17 . Navé, B.T. et al. (1999) Biochem J 344 Pt 2, 427-31. 18 . Inoki, K. et al. (2002) Nat Cell Biol 4, 648-57. 19 . Manning, B.D. et al. (2002) Mol Cell 10, 151-62. |
Western blot analysis of recombinant Akt1, Akt2 and Akt3 proteins using Akt1 (C73H10) Rabbit mAb (upper) and Akt (pan) (11E7) Rabbit mAb #4685 (lower). Western blot检测分析重组的Akt1, Akt2, Akt3蛋白 ；使用抗体是Akt1 (C73H10) Rabbit mAb 兔单抗 (上图) 和Akt (pan) (11E7) Rabbit mAb 兔单抗#4685 (下图)。 | |
Western blot analysis of extracts from Akt1 (lane 1), Akt2 (lane 2) and Akt3 (lane 3) knock-out mouse embryonic fibroblasts (MEFs) and matched wild-type MEFs (lane 4) using Akt1 (C73H10) Rabbit mAb (upper) and Akt2 Antibody #2962 (lower). Western blot检测分析敲除Akt1（第一道）, Akt2（第二道）, Akt3（第三道）的鼠胚胎成纤维细胞(MEFs)和对应的野生型MEFs（第四道）提取物；使用抗体是Akt1 (C73H10) Rabbit mAb 兔单抗(上图) 和Akt2 Antibody #2962 (下图)。 | |
Western blot analysis of extracts from various cell types using Akt1 (C73H10) Rabbit mAb. Western blot检测分析各种类型细胞提取物。使用抗体是 Akt1 (C73H10) Rabbit mAb兔单抗。 |
