| 货号 | 3011T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP |
| 目标/特异性 | PI3 Kinase p110β (C33D4) Rabbit mAb detects endogenous levels of total PI3K p110β protein. |
| 使用方法 | WB(1:1000) IP (1:25) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Phosphoinositide 3-kinase (PI3K) catalyzes the production of phosphatidylinositol-3,4,5-triphosphate by phosphorylating phosphatidylinositol (PI), phosphatidylinositol-4-phosphate (PIP) and phosphatidylinositol-4,5-bisphosphate (PIP2). Growth factors and hormones trigger this phosphorylation event, which in turn coordinates cell growth, cell cycle entry, cell migration, and cell survival (1). PTEN reverses this process, and the PI3K signaling pathway is constitutively activated in human cancers that have loss of function of PTEN (2). PI3Ks are composed of a catalytic subunit (p110) and a regulatory subunit. Various isoforms of the catalytic subunit (p110α, p110β, p110γ, and p110δ) have been isolated, and the regulatory subunits that associate with p110α, p110β, and p110δ are p85α and p85β (3). In contrast, p110γ associates with a p101 regulatory subunit that is unrelated to p85. Furthermore, p110γ is activated by βγ subunits of heterotrimeric G proteins (4).p110ß is widely distributed in tissue and plays an essential role in early embryonic development (5). p110ß stimulates cell proliferation, invasive cell growth, and expression is increased in a number of tumors including glioblastomas (6-8). |
| 存放说明 | -20C |
| 计算分子量 | 110 |
| 参考文献 | 1 . Cantley, L.C. (2002) Science 296, 1655-7. 2 . Simpson, L. and Parsons, R. (2001) Exp Cell Res 264, 29-41. 3 . Neri, L.M. et al. (2002) Biochim Biophys Acta 1584, 73-80. 4 . Stoyanov, B. et al. (1995) Science 269, 690-3. 5 . Okkenhaug, K. and Vanhaesebroeck, B. (2003) Nat Rev Immunol 3, 317-30. 6 . Czauderna, F. et al. (2003) Nucleic Acids Res 31, 670-82. 7 . Bénistant, C. et al. (2000) Oncogene 19, 5083-90. 8 . Knobbe, C.B. and Reifenberger, G. (2003) Brain Pathol 13, 507-18. |
Western blot analysis of extracts from MCF-7 and K-562 cells using PI3 Kinase p110β (C33D4) Rabbit mAb. Western blot检测分析MCF-7 和 K-562 细胞提取物。使用抗体是PI3 Kinase p110β (C33D4) Rabbit mAb兔单抗。 |
