| 货号 | 3132T |
| 同种亚型 | Rabbit |
| 反应种属 | Human/Mouse/Rat/Hamster/Monkey/Bovine |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 目标/特异性 | VASP (9A2) Rabbit mAb detects endogenous levels of total VASP protein. |
| 使用方法 | WB(1:1000) IP (1:500) IF-IC (1:400) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Vasodilator-stimulated phosphoprotein (VASP) was originally characterized as a substrate of both cGMP- and cAMP-dependent kinases (PKG and PKA, or cGPK and cAPK, respectively) (1). It is now believed that VASP belongs to the Ena/VASP family of adaptor proteins linking the cytoskeletal system to the signal transduction pathways and that it functions in cytoskeletal organization, fibroblast migration, platelet activation and axon guidance (2,3). Three phosphorylation sites, Ser157, Ser239, and Thr278, have been identified. Ser239 is the major PKG phosphorylation site while Ser157 is the major PKA phosphorylation site (4). Evidence suggests that VASP phosphorylation reduces its association with actin and has a negative effect on actin polymerization (5). Phosphorylation at Ser239 of VASP is a useful marker for monitoring PKG activation and signaling (6,7). |
| 存放说明 | -20C |
| 计算分子量 | 46, 50 |
| 参考文献 | 1 . Butt, E. et al. (1994) J Biol Chem 269, 14509-17. 2 . Ball, L.J. et al. (2000) EMBO J 19, 4903-14. 3 . Machesky, L.M. (2000) Cell 101, 685-8. 4 . Smolenski, A. et al. (1998) J Biol Chem 273, 20029-35. 5 . Harbeck, B. et al. (2000) J Biol Chem 275, 30817-25. 6 . Oelze, M. et al. (2000) Circ Res 87, 999-1005. 7 . Lawrence, D.W. and Pryzwansky, K.B. (2001) J Immunol 166, 5550-6. |
Western blot analysis of extracts from various cell lines using VASP (9A2) Rabbit mAb. | |
Immunoprecipitation of VASP from HeLa cell extracts using VASP Rabbit mAb. The same antibody was used for Western detection.使用VASP Rabbit mAb，免疫沉淀分析HeLa细胞提取物中VASP蛋白。使用同样的抗体进行免疫印迹实验。 | |
Confocal immunofluorescent analysis of HUVE cells using VASP (9A2) Rabbit mAb (green) and MEK1/2 (L38C12) Mouse mAb #4694 (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).使用VASP (9A2) Rabbit mAb (绿色)和MEK1/2 (L38C12) Mouse mAb #4694 (红色)进行免疫荧光分析HUVE细胞。蓝色= DRAQ5™ (DNA荧光染料)。 |
