| 货号 | 3180T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human,Mouse,Rat, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP , IHC-P , IF-IC , IHC-F |
| 目标/特异性 | Fatty Acid Synthase (C20G5) Rabbit mAb detects endogenous levels of total fatty acid synthase protein. |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:50) IF-IC (1:50) IHC-F (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Fatty acid synthase (FAS) catalyzes the synthesis of long-chain fatty acids from acetyl-CoA and malonyl-CoA. FAS is active as a homodimer with seven different catalytic activities and produces lipids in the liver for export to metabolically active tissues or storage in adipose tissue. In most other human tissues, FAS is minimally expressed since they rely on circulating fatty acids for new structural lipid synthesis (1). More recently, increased expression of FAS has emerged as a phenotype common to most human carcinomas. In breast cancer, immunohistochemical staining showed that the levels of FAS are directly related to the size of breast tumors (2). Studies also showed that FAS is highly expressed in lung and prostate cancers and that FAS expression is an indicator of poor prognosis in breast and prostate cancer (3-5). Furthermore, inhibition of FAS is selectively cytotoxic to human cancer cells (5). Thus, increased interest has focused on FAS as a potential target for the diagnosis and treatment of cancer as well as the metabolic syndrome (6,7). |
| 存放说明 | -20C |
| 计算分子量 | 273 |
| 参考文献 | 1 . Katsurada, A. et al. (1990) Eur J Biochem 190, 427-33. 2 . Wells, W.A. et al. (2006) Breast Cancer Res Treat 98, 231-40. 3 . Kawamura, T. et al. (2005) Pathobiology 72, 233-240. 4 . Shah, U.S. et al. (2006) Hum Pathol 37, 401-409. 5 . Kuhajda, F.P. (2000) Nutrition 16, 202-8. 6 . Tian, W.X. (2006) Curr Med Chem 13, 967-977. 7 . Kusunoki, J. et al. (2006) Endocrine 29, 91-100. |
Immunohistochemical analysis of frozen SKOV-3 xenograft using Fatty Acid Synthase (C20G5) Rabbit mAb. 对冰冻SKOV-3移植物使用Fatty Acid Synthase (C20G5) Rabbit mAb兔单抗进行免疫组化分析。 | |
Confocal immunofluorescent analysis of HeLa cells using Fatty Acid Synthase (C20G5) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye). 对HeLa细胞使用Fatty Acid Synthase (C20G5) Rabbit mAb兔单抗（绿色）进行共聚焦免疫荧光分析。肌动蛋白用DY-554 phalloidin（红色）标记。蓝色伪彩=DRAQ5?(荧光DNA染料)。 | |
Immunohistochemical analysis of paraffin-embedded hepatocellular carcinoma using Fatty Acid Synthase (C20G5) Rabbit mAb. 对石蜡包埋的肝细胞癌使用Fatty Acid Synthase (C20G5) Rabbit mAb兔单抗进行免疫组化分析。 | |
Immunohistochemical analysis of paraffin-embedded lung carcinoma using Fatty Acid Synthase (C20G5) Rabbit mAb. 对石蜡包埋的肺癌使用Fatty Acid Synthase (C20G5) Rabbit mAb兔单抗进行免疫组化分析。 | |
Immunohistochemical analysis of paraffin-embedded breast carcinoma using Fatty Acid Synthase (C20G5) Rabbit mAb. 对石蜡包埋的乳腺癌使用Fatty Acid Synthase (C20G5) Rabbit mAb兔单抗进行免疫组化分析。 | |
Immunohistochemical analysis of paraffin-embedded lymphoma, showing staining of adipocytes, using Fatty Acid Synthase (C20G5) Rabbit mAb. 对石蜡包埋的淋巴瘤使用Fatty Acid Synthase (C20G5) Rabbit mAb兔单抗进行免疫组化分析，显示脂肪细胞染色。 | |
Immunohistochemical analysis of paraffin-embedded mouse brown fat using Fatty Acid Synthase (C20G5) Rabbit mAb. 对石蜡包埋的小鼠棕色脂肪组织使用Fatty Acid Synthase (C20G5) Rabbit mAb兔单抗进行免疫组化分析。 | |
Western blot analysis of extracts from various cell types using Fatty Acid Synthase (C20G5) Rabbit mAb. 对多个细胞系使用Fatty Acid Synthase (C20G5) Rabbit mAb兔单抗进行Western blot分析。 |
