| 货号 | 3224T |
| 同种亚型 | Rabbit |
| 反应种属 | Human,Mouse,Rat,Monkey, |
| 来源宿主 | Rabbit |
| 应用 | WB, IP |
| 目标/特异性 | Dvl2 (30D2) Rabbit mAb detects endogenous levels of total Dvl2 protein. |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Dishevelled (Dsh) proteins are important intermediates of Wnt signaling pathways. Dsh inhibits glycogen synthase kinase-3β promoting β-catenin stabilization. Dsh proteins also participate in the planar cell polarity pathway by acting through JNK (1,2). There are three Dsh homologs, Dvl1, Dvl2 and Dvl3 in mammals. Upon treatment with Wnt proteins, Dvls become hyperphosphorylated (3) and accumulate in the nucleus (4). Dvl proteins also associate with actin fibers and cytoplasmic vesicular membranes (5) and mediate endocytosis of the Fzd receptor after Wnt protein stimulation (6). Overexpression of Dvl has been reported in certain cancers (7,8). |
| 存放说明 | -20C |
| 计算分子量 | 90 to 95 |
| 参考文献 | 1 . Logan, C.Y. and Nusse, R. (2004) Annu Rev Cell Dev Biol 20, 781-810. 2 . Boutros, M. and Mlodzik, M. (1999) Mech. Dev. 83, 27-37. 3 . Lee, J.S. et al. (1999) J. Biol. Chem. 274, 21464-70. 4 . Itoh, K. et al. (2005) J. Biol. 4, 3.1-3.12. 5 . Capelluto, D.G. et al. (2002) Nature 419, 726-729. 6 . Chen, W. et al. (2003) Science 301, 1391-1394. 7 . Okino , K. et al. (2003) Oncol. Rep. 10, 1219-1223. 8 . Uematsu, K. et al. (2003) Oncogene 22, 7218-7221. |
Western blot analysis of total cell lysates from Rat2 and NIH/3T3 cells, untreated or treated with conditioned medium containing Wnt3a for 60 minutes and also treated with or without calf intestinal phosphatase (CIP), using Dvl2 (30D2) Rabbit mAb.使用Dvl2 (30D2)兔单抗对未处理或经过含有Wnt3a条件培养基孵育60分钟并经过或未经过牛肠磷酸酶（CIP）处理的Rat2和NIH/3T3全细胞提取物进行western blot分析。 | |
Western blot analysis of total cell lysates from various cell lines using Dvl2 (30D2) Rabbit mAb.使用Dvl2 (30D2) 兔单抗对多种全细胞提取物进行western blotfenix。 |
