| 货号 | 3240T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human,Mouse,Monkey, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP |
| 目标/特异性 | α-E-Catenin (23B2) Rabbit mAb detects endogenous levels of total α-E catenin protein. The antibody may cross-react with neuronal α-N-catenin. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Adherens junctions are dynamic structures that form cell-cell contacts and are important in development, differentiation, tissue integrity, morphology and cell polarity. They are composed of the transmembrane proteins, cadherins, which bind cadherins on adjacent cells in a calcium-dependent manner. On the cytoplasmic side of adherens junctions, the classic model states that cadherins are linked to the cytoskeleton through β- and α-catenin. α-E-catenin is ubiquitously expressed, α-N-catenin is expressed in neuronal tissue, and α-T-catenin is primarily expressed in heart tissue. Loss of E-cadherin and α-E-catenin occurs during the progression of several human cancers, indicating that the breakdown of adherens junctions is important in cancer progression (reviewed in 1). Recent evidence suggests that, rather than acting as a static link between cadherins and actin, α-catenin regulates actin dynamics directly, possibly by competing with the actin nucleating arp2/3 complex (2,3). α-catenin also plays a role in regulating β-catenin-dependent transcriptional activity, affecting differentiation and response to Wnt signaling. α-catenin binds to β-catenin in the nucleus, preventing it from regulating transcription, and levels of both proteins appear to be regulated via proteasome-dependent degradation (4). |
| 存放说明 | -20C |
| 计算分子量 | 100 |
| 参考文献 | 1 . Kobielak, A. and Fuchs, E. (2004) Nat Rev Mol Cell Biol 5, 614-25. 2 . Yamada, S. et al. (2005) Cell 123, 889-901. 3 . Drees, F. et al. (2005) Cell 123, 903-15. 4 . Hwang, S.G. et al. (2005) J Biol Chem 280, 12758-65. |
Western blot analysis of extracts from HUVEC, NIH/3T3, MCF-7, ZR-75 and A431 cells using α-E-Catenin (23B2) Rabbit mAb.使用α-E-Catenin (23B2)兔单抗对 HUVEC, NIH/3T3, MCF-7, ZR-75 和 A431细胞提取物进行western blot分析。 |
