| 货号 | 3246T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human,Mouse,Rat,Hamster,Monkey,Dog, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB |
| 目标/特异性 | Profilin-1 (C56B8) Rabbit mAb detects endogenous levels of total profilin-1 protein. The antibody may cross-react with profilin-2. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The dynamic polymerization and depolymerization of actin filaments, a process governed by external and internal signaling events, is vital for cell motility (immune cell function, migration, invasion, metastasis, angiogenesis), cell division and adhesion. Among the many regulators of actin dynamics are profilins. Profilins are conserved actin binding proteins that affect the rate of actin polymerization by binding actin monomers and promoting the exchange of ADP for ATP (reviewed in 1). Profilins bind to proteins involved in the regulation of actin dynamics including palladin (2), dynamin-1 (3), VASP (4) and N-WASP (5). In mice, knockout of the ubiquitously expressed profilin-1 indicates that the protein is essential for embryonic development (6). Profilin-2 is primarily expressed in brain and functions in the regulation of neurite outgrowth (7), membrane trafficking and endocytosis (3). The recently cloned profilin-3 is expressed in kidney and testes (8). |
| 存放说明 | -20C |
| 计算分子量 | 15 |
| 参考文献 | 1 . Witke, W. (2004) Trends Cell Biol 14, 461-9. 2 . Boukhelifa, M. et al. (2006) FEBS J 273, 26-33. 3 . Gareus, R. et al. (2006) J Biol Chem 281, 2803-11. 4 . Reinhard, M. et al. (1995) EMBO J 14, 1583-9. 5 . Suetsugu, S. et al. (1998) EMBO J 17, 6516-26. 6 . Witke, W. et al. (2001) Proc Natl Acad Sci U S A 98, 3832-6. 7 . Da Silva, J.S. et al. (2003) J Cell Biol 162, 1267-79. 8 . Hu, E. et al. (2001) Exp Nephrol 9, 265-74. |
Western blot analysis of extracts from various cell types using Profilin-1 (C56B8) Rabbit mAb. 使用Profilin-1 (C56B8) Rabbit mAb兔单抗，免疫印迹（Western Blot）分析不同细胞中Profilin-1蛋白水平。 |
