| 货号 | 3288T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human,Mouse,Rat, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP , IF-IC |
| 目标/特异性 | EEA1 (C45B10) Rabbit mAb detects endogenous levels of total EEA1 protein. |
| 使用方法 | WB(1:1000) IP (1:100) IF-IC (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | EEA1 is an early endosomal marker and a Rab5 effector protein essential for early endosomal membrane fusion and trafficking (1-2). The carboxy terminus of EEA1 contains a FYVE domain which binds to phosphatidylinositol-3-phosphate (PtdIns(3)P), targeting EEA1 to early endosomes (3). The stable association of EEA1 with the endosomal membrane is regulated by PI3 kinase, Rab5 and calcium/calmodulin (4-6). Once on the membrane, EEA1 interacts with Rab5, NSF and syntaxin 13 to promote early endosomal membrane docking and fusion (7). |
| 存放说明 | -20C |
| 计算分子量 | 170 |
| 参考文献 | 1 . Mu, F.T. et al. (1995) J Biol Chem 270, 13503-11. 2 . Christoforidis, S. et al. (1999) Nature 397, 621-5. 3 . Gaullier, J.M. et al. (1998) Nature 394, 432-433. 4 . Patki, V. et al. (1997) Proc. Natl. Acad. Sci. USA 94, 7326-7330. 5 . Lawe, D.C. et al. (2003) Mol. Biol. Cell 14, 2935-2945. 6 . Simonsen, A. et al. (1998) Nature 394, 494-498. 7 . McBride, H.M. et al. (1999) Cell 98, 377-386. |
Western blot analysis of extracts from various cell lines using EEA1 (C45B10) Rabbit mAb. 对多个细胞系使用EEA1 (C45B10) Rabbit mAb兔单抗进行Western blot分析。 | |
Confocal immunofluorescent analysis of HeLa cells using EEA1 (C45B10) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye). 对HeLa细胞使用EEA1 (C45B10) Rabbit mAb（绿色）进行共聚焦免疫荧光分析。肌动蛋白用DY-554 phalloidin（红色）标记。蓝色伪彩=DRAQ5®(荧光DNA染料)。 |
