| 货号 | 3306T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human,Mouse,Rat,Monkey, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IF-IC |
| 目标/特异性 | G9a/EHMT2 (C6H3) Rabbit mAb detects endogenous levels of total G9a/EHMT2 protein (both the 165 kDa G9a-L and 140 kDa G9a-S isoforms). This antibody does not cross-react with other histone methyltransferases, including GLP. |
| 使用方法 | WB(1:1000) IF-IC (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | G9a, also known as Euchromatic histone-lysine N-methyltransferase 2 (EHMT2), is a member of a family of histone lysine methyltransferases, each of which contains a conserved catalytic SET domain originally identified in Drosophila Su[var]3-9, Enhancer of zeste, and Trithorax proteins (1). Recombinant G9a can mono-, di- and tri-methylate histone H3 on Lys9 and Lys27 in vitro (1,2). However, in vivo G9a forms a complex with GLP, a G9a-related histone methyltransferase, and together these proteins function as the major euchromatic histone H3 Lys9 mono- and di-methyltransferases, creating transcriptionally repressive marks that facilitate gene silencing (3,4). G9a methylates itself on Lys165, a modification that regulates the association of HP1 repressor proteins with the G9a/GLP complex (5). The G9a/GLP complex also contains Wiz, a zinc finger protein that is required for G9a/GLP hetero-dimerization and complex stability (6). Wiz contains two CtBP co-repressor binding sites, which mediate the association of the G9a/GLP with the CtBP co-repressor complex (6). In addition, G9a and GLP are components of other large transcriptional co-repressor complexes, such as those involving E2F6 and CDP/cut (7-9). G9a interacts with DNMT1, and both proteins are required for methylation of DNA and histone H3 (Lys9) at replication foci, providing a functional link between histone H3 Lys9 and CpG methylation during DNA replication (10). G9a activity is critical for meiotic prophase progression, as mutant mice deficient in germ line G9a show a large loss of mature gametes (11). In addition, G9a facilitates increased global levels of di-methyl histone H3 (Lys9) during hypoxic stress and increased G9a expression is associated with hepatocelluar carcinoma (12,13). |
| 存放说明 | -20C |
| 计算分子量 | 140, 165 |
| 参考文献 | 1 . Tachibana, M. et al. (2001) J Biol Chem 276, 25309-17. 2 . Patnaik, D. et al. (2004) J Biol Chem 279, 53248-58. 3 . Tachibana, M. et al. (2002) Genes Dev 16, 1779-91. 4 . Tachibana, M. et al. (2005) Genes Dev 19, 815-26. 5 . Sampath, S.C. et al. (2007) Mol Cell 27, 596-608. 6 . Ueda, J. et al. (2006) J Biol Chem 281, 20120-8. 7 . Ogawa, H. et al. (2002) Science 296, 1132-6. 8 . Shi, Y. et al. (2003) Nature 422, 735-8. 9 . Nishio, H. and Walsh, M.J. (2004) Proc Natl Acad Sci USA 101, 11257-62. 10 . Estève, P.O. et al. (2006) Genes Dev 20, 3089-103. 11 . Tachibana, M. et al. (2007) EMBO J 26, 3346-59. 12 . Kondo, Y. et al. (2007) Hepatol Res 37, 974-83. |
Confocal immunofluorescent analysis of HeLa cells using G9a/EHMT2 (C6H3) Rabbit mAb (green). Actin filaments have been labeled with DY-555 phalloidin (red). 使用G9a/EHMT2 (C6H3) Rabbit mAb 兔单抗(绿色)标记，共聚焦免疫荧光分析HeLa细胞。DY-554 phalloidin标记微丝蛋白(红色)。 | |
Western blot analysis of extracts from HeLa and 293 cells using G9a/EHMT2 (C6H3) Rabbit mAb. 使用G9a/EHMT2 (C6H3) Rabbit mAb兔单抗，免疫印迹(Western blot)分析HeLa和293细胞系中G9a/EHMT2的蛋白水平。 |
