| 货号 | 3358T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human,Mouse,Rat,Monkey, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB |
| 目标/特异性 | PI3 Kinase Class III (D4E2) Rabbit mAb detects endogenous levels of total PI3K class III protein. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Three distinct types of phosphoinositide 3-kinases (PI3K) have been characterized. Unlike other PI3Ks, PI3K class III catalyzes the phosphorylation of phosphatidylinositol at the D3 position, producing phosphatidylinositol-3-phosphate (PIP3) (1). PI3K class III is the mammalian homolog of Vps34, first identified in yeast. PI3K class III interacts with the regular subunit p150, the mammalian homolog of Vps15, which regulates cellular membrane association through myristoylation (2,3). PIP3 recruits several proteins with FYVE or PX domains to membranes regulating vesicular transport and protein sorting (4). Moreover, PI3K class III has been shown to regulate autophagy, trimeric G-protein signaling, and the mTOR nutrient-sensing pathway (5). |
| 存放说明 | -20C |
| 计算分子量 | 100 |
| 参考文献 | 1 . Odorizzi, G. et al. (2000) Trends Biochem Sci 25, 229-35. 2 . Panaretou, C. et al. (1997) J Biol Chem 272, 2477-85. 3 . Kihara, A. et al. (2001) J Cell Biol 152, 519-30. 4 . Corvera, S. (2001) Traffic 2, 859-66. 5 . Yan, Y. and Backer, J.M. (2007) Biochem Soc Trans 35, 239-41. |
Western blot analysis of extracts from 293 and RN33B cells using PI3 Kinase Class III (D4E2) Rabbit mAb. Western blot分析293和RN33B细胞提取物，所用抗体为PI3 Kinase Class III (D4E2) Rabbit mAb兔单抗。 |
