| 货号 | 3396T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP |
| 目标/特异性 | TCF8/ZEB1 (D80D3) Rabbit mAb detects endogenous levels of total TCF8/ZEB1 protein. |
| 使用方法 | WB(1:1000) IP (1:200) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | ZEB family proteins are zinc finger and homeobox domain containing transcription factors. There are two members in mammals, ZEB1 (δ-EF1, TCF8, AREB6) and ZEB2 (SIP1, (ZEB1 and ZEB2 contain two separate Zinc-finger domain and a homeodomain (1). While ZEB proteins mainly function as transcriptional suppressors, they are able to activate transcription, dependent on DNA-context and cell type (1). One of the targets suppressed by ZEB proteins is E-cadherin. Downregulation of E-cadherin is one of the hallmarks of epithelial mesenchymal transition (EMT), a critical feature of normal embryonic development, which is also utilized by malignant epithelial tumors to spread beyond their origin (2-4). ZEB1 mutations are associated with posterior corneal dystrophy, and ZEB2 mutations were reported to be associated with Hirschsprung (HSCR) disease (5-8). |
| 存放说明 | -20C |
| 计算分子量 | 200 |
| 参考文献 | 1 . Vandewalle, C. et al. (2009) Cell Mol Life Sci 66, 773-87. 2 . Aigner, K. et al. (2007) Oncogene 26, 6979-88. 3 . Peinado, H. et al. (2007) Nat Rev Cancer 7, 415-28. 4 . Moreno-Bueno, G. et al. (2008) Oncogene 27, 6958-69. 5 . Krafchak, C.M. et al. (2005) Am J Hum Genet 77, 694-708. 6 . Aldave, A.J. et al. (2007) Am J Med Genet A 143A, 2549-56. 7 . Dastot-Le Moal, F. et al. (2007) Hum Mutat 28, 313-21. 8 . Garavelli, L. and Mainardi, P.C. (2007) Orphanet J Rare Dis 2, 42. |
Western blot analysis of extracts from Jurkat, HT1080 and A172 cells using TCF8/ZEB1 (D80D3) Rabbit mAb. 使用TCF8/ZEB1 (D80D3)Rabbit mAb兔单抗对Jurkat，HT1080和A172细胞提取物进行western blot分析。 | |
Western blot analysis of extracts from COS cells, mock transfected or transfected with a construct expressing human TCF8/ZEB1, using TCF8/ZEB1 (D80D3) Rabbit mAb. 使用TCF8/ZEB1 (D80D3)Rabbit mAb兔单抗对未转染或转染了人TCF8/ZEB1表达质粒的COS细胞提取物进行western blot分析。 |
