| 货号 | 3629T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human,Mouse,Rat,Monkey, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP , IF-IC |
| 目标/特异性 | Integrin beta-5 (D24A5) Rabbit mAb detects endogenous levels of total integrin beta-5 protein. |
| 使用方法 | WB(1:1000) IP (1:50) IF-IC (1:1600) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Integrins are α/β heterodimeric cell surface receptors that play a pivotal role in cell adhesion and migration, as well as in growth and survival (1,2). The integrin family contains at least 18 α and 8 β subunits that form 24 known integrins having distinct tissue distribution and overlapping ligand specificities (3). Integrins not only transmit signals to cells in response to the extracellular environment (outside-in signaling), but also sense intracellular cues to alter their interaction with extracellular environment (inside-out signaling) (1,2). The αVβ5 integrin is expressed in various tissues and cell types, including endothelia, epithelia and fibroblasts (4). It plays a role in matrix adhesion to VN, FN, SPARC and bone sialoprotein (5) and functions in the invasion of gliomas and metastatic carcinoma cells (6,7). αVβ5 integrin plays a major role in growth-factor-induced tumor angiogenesis, where cooperative signaling by the αVβ5 integrin and growth factors regulates endothelial cell proliferation and survival (8). |
| 存放说明 | -20C |
| 计算分子量 | 90 |
| 参考文献 | 1 . Hood, J.D. and Cheresh, D.A. (2002) Nat Rev Cancer 2, 91-100. 2 . Liu, S. et al. (2000) J Cell Sci 113 ( Pt 20), 3563-71. 3 . van der Flier, A. and Sonnenberg, A. (2001) Cell Tissue Res 305, 285-98. 4 . Pasqualini, R. et al. (1993) J. Cell Sci. 105, 101-111. 5 . Plow, E.F. et al. (2000) J Biol Chem 275, 21785-8. 6 . Sun, L.C. et al. (2007) Cancer Lett. 246, 157-166. 7 . Enns, A. et al. (2005) Eur. J. Cancer 41, 1065-1072. 8 . Alghisi, G.C. and Rüegg, C. (2006) Endothelium 13, 113-135. |
Western blot analysis of extracts from various cell lines using Integrin beta-5 (D24A5) Rabbit mAb. | |
Confocal immunofluorescent analysis of A549 cells using Integrin β5 (D24A5) Rabbit mAb (green). Actin filaments were labeled using DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). |
