| 货号 | 7065T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP |
| 目标/特异性 | c-IAP1 (D5G9) Rabbit mAb recognizes endogenous levels of total c-IAP1 protein. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The inhibitor of apoptosis protein (IAP) family consists of an evolutionarily conserved group of apoptosis inhibitors containing a conserved 70 amino acid BIR (baculovirus inhibitor repeat) domain (1,2). Human members of this family include c-IAP1, c-IAP2, XIAP, survivin, livin, and NAIP. Overexpression of IAP family members, particularly survivin and livin, in cancer cell lines and primary tumors suggests an important role for these proteins in cancer progression (3-5). In general, the IAP proteins function through direct interactions to inhibit the activity of several caspases, including caspase-3, caspase-7, and caspase-9 (5,6). In addition, binding of IAP family members to the mitochondrial protein Smac blocks their interaction with caspase-9, thereby allowing the processing and activation of the caspase (2). |
| 存放说明 | -20C |
| 计算分子量 | 62 |
| 参考文献 | 1 . Deveraux, Q.L. and Reed, J.C. (1999) Genes Dev 13, 239-52. 2 . Deveraux, Q. L. et al. (1998) EMBO J. 17, 2215-2223. 3 . Altieri, D.C. et al. (1999) Lab Invest 79, 1327-33. 4 . Tamm, I. et al. (2000) Clin Cancer Res 6, 1796-803. 5 . Kasof, G.M. and Gomes, B.C. (2001) J Biol Chem 276, 3238-46. 6 . Deveraux, Q.L. et al. (1997) Nature 388, 300-4. |
Western blot analysis of extracts from HeLa cells, mock transfected (-) or transfected with human c-IAP1 (+), using c-IAP1 (D5G9) Rabbit mAb. Western blot方法检测293细胞提取物，空质粒（-）或人 c-IAP1转染 (+)；使用抗体为c-IAP1 (D5G9) Rabbit mAb 兔单抗。 | |
Western blot analysis of extracts from various cell lines using c-IAP1 (D5G9) Rabbit mAb. Western blot 检测多种细胞系提取物，使用抗体为c-IAP1 (D5G9) Rabbit mAb 兔单抗。 |
