| 货号 | 3845T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human,Monkey, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, F |
| 目标/特异性 | LIMK2 (8C11) Rabbit Monoclonal Antibody detects endogenous levels of total LIMK2 protein. The antibody does not cross-react with LIMK1. |
| 使用方法 | WB(1:1000) F (1:200) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | LIM kinases (LIMK1 and LIMK2) are serine/threonine kinases that have two zinc finger motifs, known as LIM motifs, in their amino-terminal regulatory domains (1). LIM kinases are involved in actin cytoskeletal regulation downstream of Rho-family GTPases, PAKs and ROCK (2,3). PAK1 and ROCK phosphorylate LIMK1 or LIMK2 at the conserved Thr508 or Thr505 residues in the activation loop, increasing the LIMK activity (3-5). Activated LIM kinases inhibit the actin depolymerization activity of cofilin by phosphorylation at the amino-terminal Ser3 residue of cofilin (6,7). |
| 存放说明 | -20C |
| 计算分子量 | 70 |
| 参考文献 | 1 . Okano, I. et al. (1995) J Biol Chem 270, 31321-30. 2 . Maekawa, M. et al. (1999) Science 285, 895-8. 3 . Edwards, D.C. et al. (1999) Nat Cell Biol 1, 253-9. 4 . Ohashi, K. et al. (2000) J Biol Chem 275, 3577-82. 5 . Sumi, T. et al. (2001) J Biol Chem 276, 670-6. 6 . Arber, S. et al. (1998) Nature 393, 805-809. 7 . Yang, N. et al. (1998) Nature 393, 809-812. |
Western blot analysis of extracts from Colo201 and Jurkat cells, using LIMK2 (8C11) Rabbit mAb. 使用LIMK2 (8C11) Rabbit mAb，免疫印迹（Western Blot）分析Colo201和Jurkat细胞LIMK2蛋白水平。 | |
Flow cytometric analysis of Jurkat cells, using LIMK2 (8C11) Rabbit mAb Antibody (blue) compared to a nonspecific negative control antibody (red). 与非特异阴性control antibody (红色)比较，使用LIMK2 (8C11) Rabbit mAb Antibody(蓝色)标记，流式细胞仪分析Jurkat细胞。 |
