| 货号 | 9532T |
| 同种亚型 | Rabbit |
| 反应种属 | Human,Mouse,Rat,Monkey, |
| 来源宿主 | Rabbit |
| 应用 | WB, IP , IF-IC |
| 目标/特异性 | PARP (46D11) Rabbit mAb detects endogenous levels of total full-length PARP and the large fragment (89 kDa) produced by caspase cleavage. |
| 使用方法 | WB(1:1000) IP (1:200) IF-IC (1:800) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress (1). This protein can be cleaved by many ICE-like caspases in vitro (2,3) and is one of the main cleavage targets of caspase-3 in vivo (4,5). In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa) (2,4). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (6). |
| 存放说明 | -20C |
| 计算分子量 | 116, 89 |
| 参考文献 | 1 . Satoh, M.S. and Lindahl, T. (1992) Nature 356, 356-358. 2 . Lazebnik, Y. A. et al. (1994) Nature 371, 346-347. 3 . Cohen, G.M. (1997) Biochem. J. 326, 1-16. 4 . Nicholson, D. W. et al. (1995) Nature 376, 37-43. 5 . Tewari, M. et al. (1995) Cell 81, 801-809. 6 . Oliver, F.J. et al. (1998) J. Biol. Chem. 273, 33533-33539. |
Confocal immunofluorescent analysis of untreated HeLa cells labeled with PARP (46D11) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red).采用PARP (46D11) Rabbit mAb对未处理的Hela细胞进行共聚焦免疫荧光分析。微丝用Alexa Fluor® 555鬼比环肽（红色）标记。 | |
Western blot analysis of extracts from THP-1 cells, untreated or treated with TNF-α and cycloheximide as well as control extracts from SW620 and A20 cell lines, using PARP (46D11) Rabbit mAb.采用PARP (46D11) Rabbit mAb对未处理或TNF-α，放线菌酮处理的THP-1细胞进行免疫印迹分析，同时用SW620 and A20 细胞系作对照。 |
