Atg5 (D5F5U) Rabbit mAb【科瑞奥博】

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Atg5 (D5F5U) Rabbit mAb

货号： 12994T 基本售价： 1350.0 元规格： -

产品信息

概述
货号	12994T
同种亚型	Rabbit IgG
反应种属	Human,Mouse,Rat,
来源宿主	Rabbit IgG
应用	WB, IP
目标/特异性	Atg5 (D5F5U) Rabbit mAb recognizes endogenous levels of total Atg5 protein. This antibody is capable of detecting Atg5 conjugated to Atg12.
使用方法	WB(1:1000) IP (1:100)

性能
供应商	CST
灵敏度	Endogenous
背景
存放说明	-20C
计算分子量	55
参考文献	1 . Reggiori, F. and Klionsky, D.J. (2002) Eukaryot Cell 1, 11-21. 2 . Codogno, P. and Meijer, A.J. (2005) Cell Death Differ 12 Suppl 2, 1509-18. 3 . Levine, B. and Yuan, J. (2005) J Clin Invest 115, 2679-88. 4 . Mizushima, N. et al. (1998) J Biol Chem 273, 33889-92. 5 . Mizushima, N. et al. (1998) Nature 395, 395-8. 6 . Suzuki, K. et al. (2001) EMBO J 20, 5971-81. 7 . Tanida, I. et al. (1999) Mol Biol Cell 10, 1367-79. 8 . Shintani, T. et al. (1999) EMBO J 18, 5234-41.

参考图片

Western blot analysis of extracts from various cell lines using Atg5 (D5F5U) Rabbit mAb.

Western blot 分析不同细胞系的提取物，使用的抗体是Atg5 (D5F5U) Rabbit mAb 兔单抗。

Western blot analysis of extracts from wild-type MEFs (wt) or MEFs from Atg5 knockouts (Atg5^-/-) using Atg5 (D5F5U) Rabbit mAb (upper), or β-Actin (D6A8) Rabbit mAb #8457 (lower). Atg5^-/- MEFs were kindly provided by Dr. Ramnik Xavier, Massachusetts General Hospital, Harvard Medical School, Boston, MA.

Immunoprecipitation of Atg5 from PANC-1 cell extracts using Rabbit (DA1E) mAb IgG XP^® Isotype Control #3900 (lane 2) or Atg5 (D5F5U) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot was performed using Atg5 (D5F5U) Rabbit mAb. Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb #3678 was used as a secondary antibody to avoid cross-reactivity with rabbit IgG.

免疫沉淀分析PANC-1细胞中的Atg5，使用的抗体是Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (泳道 2) 或 Atg5 (D5F5U) Rabbit mAb 兔单抗 (泳道 3)。泳道1是10% input，Western blot 使用的是Atg5 (D5F5U) Rabbit mAb 兔单抗。鼠源Anti-rabbit IgG (Conformation Specific) (L27A9) mAb #3678作为二抗以避免与兔源IgG发生交叉反应。