| 货号 | 4035T |
| 同种亚型 | Rabbit |
| 反应种属 | Human,Mouse,Rat,Monkey, |
| 来源宿主 | Rabbit |
| 应用 | WB, IP |
| 目标/特异性 | ROCK1 (C8F7) Rabbit mAb detects endogenous levels of total ROCK1 protein. |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | ROCK (Rho-associated kinase), a family of serine/threonine kinases, is an important downstream target of GTPase Rho and plays an important role in Rho-mediated signaling. Two isoforms of ROCK have been identified (ROCK1 and ROCK2). ROCK is composed of N-terminal catalytic, coiled-coil, and C-terminal PH (pleckstrin homology) domains. The C-terminus of ROCK negatively regulates its kinase activity (1,2). Caspase-3-induced cleavage of ROCK1 and direct cleavage of ROCK2 by granzyme B (grB) activates ROCK and leads to phosphorylation of myosin light chain and inhibition of myosin phosphatase (3). This phosphorylation may account for the mechanism by which Rho regulates cytokinesis, cell motility, cell membrane blebbing during apoptosis, and smooth muscle contraction (4-6). |
| 存放说明 | -20C |
| 计算分子量 | 160 |
| 参考文献 | 1 . Nakagawa, O. et al. (1996) FEBS Lett. 392, 189-193. 2 . Lee, J.H. et al. (2004) J. Cell. Biol. 167, 327-337. 3 . Sebbagh, M. et al. (2005) J. Exp. Med. 201, 465-471. 4 . Amano, M. et al. (1996) J. Biol. Chem. 271, 20246-20249. 5 . Kureishi, Y. et al. (1997) J. Biol. Chem. 272, 12257-12260. 6 . Totsukawa, G. et al. (2000) J. Cell Biol. 150, 797-806. |
Immunoprecipitation of ROCK1 from HeLa cell extracts using ROCK1 (C8F7) Rabbit mAb. Western blot detection was perfomed using the same antibody. Lane 1 is 5% input. 使用ROCK1 (C8F7) Rabbit mAb，免疫沉淀分析HeLa细胞ROCK1蛋白。使用同样抗体进行免疫印迹（Western Blot）实验。第1道是5% input。 | |
Western blot analysis of extracts from various cell types using ROCK1 (C8F7) Rabbit mAb. 使用ROCK1 (C8F7) Rabbit mAb，免疫印迹（Western Blot）分析不同细胞中ROCK1蛋白水平。 |
