| 货号 | 3136T |
| 同种亚型 | Mouse IgG1 |
| 反应种属 | Human,Mouse,Rat, |
| 来源宿主 | Mouse IgG1 |
| 应用 | WB |
| 目标/特异性 | CDK6 (DCS83) Mouse mAb detects endogenous levels of total CDK6 protein. |
| 使用方法 | WB(1:2000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The cyclin-dependent kinases form complexes with their cyclin partners and with CDK inhibitors. CDK6 and CDK4 associate with the D-type cyclins and target the retinoblastoma protein, allowing passage through the G1/S phase restriction point (1). CDK6/cyclin D complexes are sequestered in their inactive form through binding to one of the INK4 CDK inhibitor family members (2,3). Unlike the INK4 family of cdk inhibitors, the CDK inhibitor p21 Waf1/Cip1 may enhance the association of CDK4 and CDK6 with their cyclin D partners (4).周期暗白依赖性激酶和其蛋白伴侣和CDK抑制剂共同组成复合体。CDK6 和CDK4协同D-型周期蛋白并靶向Rb蛋白，促进G1/S期限制点的通过(1)。CDK6/cyclin D复合体的失活状态是游离的，通过结合INK4 CDK抑制剂家族的成员来实现(2,3)。与cdk抑制剂INK4家族不同，CDK抑制剂p21 Waf1/Cip1可以增强CDK4和CDK6及其与周期蛋白D伴侣的缔合(4)。 |
| 存放说明 | -20C |
| 计算分子量 | 36 |
| 参考文献 | 1 . Lukas, J. et al. (1996) Mol Cell Biol 16, 6917-25. 2 . Hirai, H. et al. (1995) Mol Cell Biol 15, 2672-81. 3 . Pestell, R.G. et al. (1999) Endocr Rev 20, 501-34. 4 . Flores-Rozas, H. et al. (1994) Proc Natl Acad Sci U S A 91, 8655-9. |
Western blot analysis of extracts from HeLa, IM-CD-3 and C6 cells, using CDK6 (DCS83) Mouse mAb.western blot方法检测HeLa, IM-CD-3 和C6细胞提取物，使用的抗体为CDK6 (DCS83) Mouse mAb。 |
