| 货号 | 4151T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human,Mouse,Rat,Monkey, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB |
| 目标/特异性 | ADAM9 (D64B5) Rabbit mAb detects endogenous levels of total ADAM9 protein. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The ADAM (A Disintegrin and A Metalloprotease) family of multidomain membrane proteins influences cell signaling and adhesion by shedding cell surface proteins such as cytokines and growth factors, by influencing cell adhesion to the extracellular matrix (ECM), and by directly remodeling the ECM. Conserved domains in ADAM family members include a prodomain, a zinc-dependent metalloprotease domain, a disintegrin domain, a cysteine-rich domain, an EGF-like sequence and a short cytoplasmic tail (1,2). The prodomain is thought to aid in protein folding. Disintegrin and cysteine-rich domains mediate adhesion at least in part through binding to integrins. Phosphorylation of the cytoplasmic tail as well as its interaction with other signaling proteins may influence intra- and extracellular signaling (1). ADAM9 is widely distributed and has been shown to affect migration in skin keratinocytes (3,4). ADAM9 is overexpressed in prostate cancer (5), pancreatic cancer (6), gastric cancer (7) and has been linked to invasion and metastasis in small cell lung cancer (8). An alternatively spliced short (50 kDa) form of ADAM9 containing protease activity is involved in tumor cell invasion (9). |
| 存放说明 | -20C |
| 计算分子量 | 100-115, 75-80 |
| 参考文献 | 1 . N. M. Hooper and U. Lendeckel. . The Netherlands: Springer, 2005 2 . Schlöndorff, J. and Blobel, C.P. (1999) J Cell Sci 112 ( Pt 21), 3603-17. 3 . Franzke, C.W. et al. (2002) EMBO J 21, 5026-35. 4 . Zigrino, P. et al. (2007) J Biol Chem 282, 30785-93. 5 . Fritzsche, F.R. et al. (2008) Eur Urol 54, 1097-106. 6 . Grützmann, R. et al. (2004) Br J Cancer 90, 1053-8. 7 . Carl-McGrath, S. et al. (2005) Int J Oncol 26, 17-24. 8 . Shintani, Y. et al. (2004) Cancer Res 64, 4190-6. 9 . Mazzocca, A. et al. (2005) Cancer Res 65, 4728-38. |
Western blot analysis of extracts from various cell types using ADAM9 (D64B5) Rabbit mAb.使用ADAM9 (D64B5) 兔单抗对多种细胞提取物进行western blot分析。 |
