| 货号 | 4257T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human,Mouse,Rat, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP |
| 目标/特异性 | PI3 Kinase p85 (19H8) Rabbit mAb detects endogenous levels of total PI3K p85 protein. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Phosphoinositide 3-kinase (PI3K) catalyzes the production of phosphatidylinositol-3,4,5-triphosphate by phosphorylating phosphatidylinositol (PI), phosphatidylinositol-4-phosphate (PIP) and phosphatidylinositol-4,5-bisphosphate (PIP2). Growth factors and hormones trigger this phosphorylation event, which in turn coordinates cell growth, cell cycle entry, cell migration, and cell survival (1). PTEN reverses this process, and the PI3K signaling pathway is constitutively activated in human cancers that have loss of function of PTEN (2). PI3Ks are composed of a catalytic subunit (p110) and a regulatory subunit. Various isoforms of the catalytic subunit (p110α, p110β, p110γ, and p110δ) have been isolated, and the regulatory subunits that associate with p110α, p110β, and p110δ are p85α and p85β (3). In contrast, p110γ associates with a p101 regulatory subunit that is unrelated to p85. Furthermore, p110γ is activated by βγ subunits of heterotrimeric G proteins (4). |
| 存放说明 | -20C |
| 计算分子量 | 85 |
| 参考文献 | 1 . Cantley, L.C. (2002) Science 296, 1655-7. 2 . Simpson, L. and Parsons, R. (2001) Exp Cell Res 264, 29-41. 3 . Neri, L.M. et al. (2002) Biochim Biophys Acta 1584, 73-80. 4 . Stoyanov, B. et al. (1995) Science 269, 690-3. |
Western blot analysis of extracts from Jurkat and NIH/3T3 cells, using PI3 Kinase p85 (19H8) Rabbit mAb. Western blot分析Jurkat 和NIH/3T3细胞的提取物，所使用的是PI3 Kinase p85 (19H8) Rabbit mAb兔单抗。 |
