| 货号 | 4266T |
| 同种亚型 | Mouse IgG1 |
| 反应种属 | Human, |
| 来源宿主 | Mouse IgG1 |
| 应用 | WB, IP |
| 目标/特异性 | SOD1 (71G8) Mouse mAb detects endogenous levels of SOD1 protein. It does not cross-react with other related proteins. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | SOD1, Cu/Zn superoxide dismutase, is a major antioxidant enzyme that catalyzes the conversion of superoxide anion to hydrogen peroxide and molecular oxygen (1). SOD1 is ubiquitously expressed and is localized in the cytosol, nucleus and mitochondrial intermembrane space. The SOD1 gene locus is on chromosome 21 in a region affected in Down Syndrome (2). In addition, over 100 distinct SOD1 inherited mutations have been identified in the familial form of amyotrophic lateral sclerosis (ALS), a progressive degenerative disease of motor neurons (3-5). Despite the fact that SOD1 helps to eliminate toxic reactive species, its mutations in ALS have been described as gain-of-function (5). The mechanism by which mutant SOD1 induces the neurodegeneration observed in ALS is still unclear. Mutant SOD1 proteins become misfolded and consequently oligomerize into high molecular weight species that aggregate and end up in proteinaceous inclusions (5). |
| 存放说明 | -20C |
| 计算分子量 | 18 |
| 参考文献 | 1 . McCord, J.M. and Fridovich, I. (1988) Free Radic Biol Med 5, 363-9. 2 . Sherman, L. et al. (1983) Proc Natl Acad Sci U S A 80, 5465-9. 3 . Rosen, D.R. et al. (1993) Nature 362, 59-62. 4 . Deng, H.X. et al. (1993) Science 261, 1047-51. 5 . Valentine, J.S. and Hart, P.J. (2003) Proc Natl Acad Sci USA 100, 3617-22. |
Western blot analysis of extracts from various cell lines using SOD1 (71G8) Mouse mAb. Western blot分析多个细胞系提取物，使用的抗体是SOD1 (71G8) Mouse mAb 鼠单抗。 |
