| 货号 | 9665T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human,Mouse,Rat,Monkey, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP |
| 目标/特异性 | Caspase-3 (8G10) Rabbit mAb detects endogenous levels of full-length (35 kDa) and large fragment (17/19 kDa) of caspase-3 resulting from cleavage at aspartic acid 175. |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Caspase-3 (CPP-32, Apoptain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins such as the nuclear enzyme poly(ADP-ribose) polymerase (PARP) (1). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires aspartic acid at the P1 position (2). |
| 存放说明 | -20C |
| 计算分子量 | 17, 19, 35 |
| 参考文献 | 1 . Fernandes-Alnemri, T. et al. (1994) J Biol Chem 269, 30761-4. 2 . Nicholson, D. W. et al. (1995) Nature 376, 37-43. |
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 (-) or SignalSilence® Caspase-3 siRNA II (+), using Caspase-3 (8G10) Rabbit mAb and α-Tubulin (11H10) Rabbit mAb #2125. Caspase-3 (8G10) Rabbit mAb confirms silencing of caspase-3 expression, while the α-Tubulin (11H10) Rabbit mAb is used to control for loading and specificity of caspase-3 siRNA. 免疫印记（western blot）分析转染了100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 (-)或者SignalSilence® Caspase-3 siRNA I (+) 的Hela细胞，采用 Caspase-3 (8G10) Rabbit mAb 兔单抗，Caspase-3 (8G10) Rabbit mAb 兔单抗证实了caspas-3表达的沉默， 而α-Tubulin (11H10) Rabbit mAb 兔单抗#2125 抗体作为 caspase-3 siRNA上样量及特异性的对照。 | |
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 (-) or SignalSilence® Caspase-3 siRNA I (+), using Caspase-3 (8G10) Rabbit mAb and p42 MAPK Antibody #9108. Caspase-3 (8G10) Rabbit mAb confirms silencing of caspase-3 expression, while the p42 MAPK Antibody is used to control for loading and specificity of caspase-3 siRNA. 免疫印记（western blot）分析转染了100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 (-)或者SignalSilence® Caspase-3 siRNA I (+) 的Hela细胞，采用 Caspase-3 (8G10) Rabbit mAb 兔单抗。Caspase-3 (8G10) Rabbit mAb 兔单抗证实了caspas-3表达的沉默， p42 MAPK 抗体作为 caspase-3 siRNA 特异性的对照。 | |
Immunoprecipitation of cleaved caspase-3 from Jurkat cell extracts untreated (control) or treated with etoposide (25uM 5hrs) (apoptotic) using Caspase-3 (8G10) Rabbit mAb, and western probed with the same antibody. 免疫沉淀分析未处理与 etoposide （依托泊苷）处理（25uM 5hrs）的Jurkat细胞，然后采用Caspase-3 (8G10) Rabbit mAb 兔单抗进行免疫印迹分析 | |
Western analysis of HeLa (human) and NIH/3T3 (mouse) cell extracts, untreated and treated with 1 uM staurosporine (3hrs) in vivo, using Caspase-3 (8G10) Rabbit mAb. 采用Caspase-3 (8G10) Rabbit mAb 兔单抗对未处理和 staurosporine（1uM，3hrs）刺激的人HeLa细胞和老鼠NIH/3T3 细胞进行免疫印迹分析（western blot）。 |
