| 货号 | 4558T |
| 同种亚型 | Mouse IgG1 |
| 反应种属 | Human, |
| 来源宿主 | Mouse IgG1 |
| 应用 | WB, IHC-P |
| 目标/特异性 | Keratin 19 (BA17) Mouse mAb detects endogenous levels of total keratin 19 protein. The antibody does not cross-react with other keratin proteins. |
| 使用方法 | WB(1:1000) IHC-P (1:200) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Keratins (cytokeratins) are intermediate filament proteins that are mainly expressed in epithelial cells. Keratin heterodimers composed of an acidic keratin (or type I keratin, keratins 9 to 23) and a basic keratin (or type II keratin, keratins 1 to 8) assemble to form filaments (1,2). Keratin isoforms demonstrate tissue- and differentiation-specific profiles that make them useful as biomarkers (1). Mutations in keratin genes are associated with skin disorders, liver and pancreatic diseases, and inflammatory intestinal diseases (3-6). |
| 存放说明 | -20C |
| 计算分子量 | 40 |
| 参考文献 | 1 . Moll, R. et al. (1982) Cell 31, 11-24. 2 . Chang, L. and Goldman, R.D. (2004) Nat Rev Mol Cell Biol 5, 601-13. 3 . Ramaekers, F.C. and Bosman, F.T. (2004) J Pathol 204, 351-4. 4 . Lane, E.B. and McLean, W.H. (2004) J Pathol 204, 355-66. 5 . Zatloukal, K. et al. (2004) J Pathol 204, 367-76. 6 . Owens, D.W. and Lane, E.B. (2004) J Pathol 204, 377-85. |
Immunhistochemical analysis of paraffin-embedded human breast carcinoma using Keratin 19 (BA17) Mouse mAb.使用Keratin 19 (BA17) Mouse mAb，免疫组化分析人源乳腺癌组织石蜡切片。 | |
Immunohistochemical analysis of paraffin-embedded MCF-7 cells (positive, left) and A-431 cells (negative, right), using Keratin 19 (BA17) Mouse mAb.使用Keratin 19 (BA17) Mouse mAb，免疫组化分析MCF-7细胞(阳性, 左图)和A-431 细胞(阴性,右图)石蜡切片。 | |
Western blot analysis of extracts from various cell types using Keratin 19 (BA17) Mouse mAb. As expected, the protein is absent in A-431 and HeLa cells.使用Keratin 19 (BA17) Mouse mAb，免疫印迹（Western Blot）分析不同细胞中Keratin 19蛋白水平。说明：正如预期的A-431和HeLa细胞中未检测到该蛋白。 |
