| 货号 | 4657T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP |
| 目标/特异性 | Vav1 (D45G3) Rabbit mAb recognizes endogenous levels of total Vav1 protein. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Vav proteins belong to the Dbl family of guanine nucleotide exchange factors (GEFs) for Rho/Rac small GTPases. The three identified mammalian Vav proteins (Vav1, Vav2 and Vav3) differ in their expression. Vav1 is expressed only in hematopoietic cells and is involved in the formation of the immune synapse. Vav2 and Vav3 are more ubiquitously expressed. Vav proteins contain the Dbl homology domain, which confers GEF activity, as well as protein interaction domains that allow them to function in pathways regulating actin cytoskeleton organization (reviewed in 1). Phosphorylation stimulates the GEF activity of Vav protein towards Rho/Rac (2,3). |
| 存放说明 | -20C |
| 计算分子量 | 95 |
| 参考文献 | 1 . Hornstein, I. et al. (2004) Cell. Signal. 16, 1-11. 2 . Crespo, P. et al. (1997) Nature 385, 169-172. 3 . Han, J. et al. (1997) Mol. Cell. Biol. 17, 1346-1353. |
Immunoprecipitation of Vav1 from Jurkat cells using Vav1 (D45G3) Rabbit mAb. Western blot was performed using the same antibody. 使用Vav1 (D45G3) Rabbit mAb兔单抗，免疫沉淀分析Jurkat细胞裂解物中Vav1蛋白。使用同样的抗体进行免疫印迹实验。 | |
Western blot analysis of extracts from Jurkat, Raji and K-562 cells using Vav1 (D45G3) Rabbit mAb. 使用Vav1 (D45G3) Rabbit mAb兔单抗，免疫印迹(Western blot)分析Jurkat、Raji和K-562细胞中Vav1蛋白水平。 |
