| 货号 | 4695T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human,Mouse,Rat,Hamster,Monkey,Mink,D. melanogaster,Zebrafish,Bovine,Dog,Pig,C. elegans, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP , IHC-P , F , IF-IC |
| 目标/特异性 | p44/42 MAP Kinase (137F5) Rabbit mAb detects endogenous levels of total p44/42 MAP kinase (Erk1/Erk2) protein. The antibody does not cross-react with JNK/SAPK or p38 MAP kinase. |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:250) F (1:400) IF-IC (1:800) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines (1-3) and is an important target in the diagnosis and treatment of cancer (4). Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44/42 MAP3Ks have been identified, including members of the Raf family as well as Mos and Tpl2/Cot. MEK1 and MEK2 are the primary MAPKKs in this pathway (5,6). MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK (7) and the transcription factor Elk-1 (8,9). p44/42 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases, known as DUSPs or MKPs (10), along with MEK inhibitors such as U0126 and PD98059. |
| 存放说明 | -20C |
| 计算分子量 | 42, 44 |
| 参考文献 | 1 . Roux, P.P. and Blenis, J. (2004) Microbiol Mol Biol Rev 68, 320-44. 2 . Baccarini, M. (2005) FEBS Lett 579, 3271-7. 3 . Meloche, S. and Pouysségur, J. (2007) Oncogene 26, 3227-39. 4 . Roberts, P.J. and Der, C.J. (2007) Oncogene 26, 3291-310. 5 . Rubinfeld, H. and Seger, R. (2005) Mol Biotechnol 31, 151-74. 6 . Murphy, L.O. and Blenis, J. (2006) Trends Biochem Sci 31, 268-75. 7 . Dalby, K.N. et al. (1998) J Biol Chem 273, 1496-505. 8 . Marais, R. et al. (1993) Cell 73, 381-93. 9 . Kortenjann, M. et al. (1994) Mol Cell Biol 14, 4815-24. 10 . Owens, D.M. and Keyse, S.M. (2007) Oncogene 26, 3203-13. 11 . Suzuki, M. et al. (2015) J Immunol 195, 1273-81. |
Western blot analysis of extracts from HeLa, NIH/3T3 and C6 cells, using p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb. | |
Western blot方法检测细胞裂解物：HeLa, NIH/3T3，C6细胞。使用的抗体是p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb兔单抗。 | |
Flow cytometric analysis of Jurkat cells, U0126-treated (blue) or PMA-treated (green), using p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb compared to a nonspecific negative control antibody (red). | |
免疫组织化学方法检测石蜡包埋的人结肠癌组织。使用的抗体为p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb兔单抗。 | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb. | |
免疫组织化学方法检测石蜡包埋的人乳腺癌组织，图中显示了细胞核、细胞质的位置。使用的抗体为p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb兔单抗。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing cytoplasmic and nuclear localization, using p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb. |
