| 货号 | 4715T |
| 同种亚型 | Rabbit |
| 反应种属 | Human, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP , IHC-P , F , IF-IC |
| 目标/特异性 | TRAF1 (45D3) Rabbit mAb detects endogenous levels of total TRAF1 protein. |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:100) F (1:200) IF-IC (1:800) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | TRAFs (TNF receptor-associated factors) are a family of multifunctional adaptor proteins that bind to surface receptors and recruit additional proteins to form multiprotein signaling complexes capable of promoting cellular responses (1-3). Members of the TRAF family share a common carboxy-terminal "TRAF domain" which mediates interactions with associated proteins; many also contain amino-terminal Zinc/RING finger motifs. The first TRAFs identified, TRAF1 and TRAF2, were found by virtue of their interactions with the cytoplasmic domain of TNF-receptor 2 (TNFRII) (4). The six known TRAFs (TRAF1-6) act as adaptor proteins for a wide range of cell surface receptors and participate in the regulation of cell survival, proliferation, differentiation, and stress responses. |
| 存放说明 | -20C |
| 计算分子量 | 50 |
| 参考文献 | 1 . Arch, R.H. et al. (1998) Genes Dev 12, 2821-30. 2 . Chung, J.Y. et al. (2002) J Cell Sci 115, 679-88. 3 . Bradley, J.R. and Pober, J.S. (2001) Oncogene 20, 6482-91. 4 . Rothe, M. et al. (1994) Cell 78, 681-92. |
Confocal immunofluorescent analysis of HT-1080 (upper) and HEK/293 (lower) cells, untreated (left) or treated with TNF-α #8902 (right), using TRAF1 (45D3) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 共聚焦免疫荧光分析HT-1080（上图）和HEK/293（下图）细胞，未处理（左）或TNF-α #8902（右）处理，使用抗体为TRAF1 (45D3) Rabbit mAb 兔单抗（绿色）。DY-554 phalloidin（红色）标记肌动蛋白丝。蓝色伪彩=DRAQ5®＃4084（荧光DNA染料）。 | |
Immunohistochemical analysis of paraffin-embedded human tonsil using TRAF1 (45D3) Rabbit mAb preincubated with a control peptide (left) or TRAF1 Blocking Peptide #1066 (right). 免疫组织化学分析石蜡包埋的人扁桃体切片，对照肽（左）或TRAF1 Blocking Peptide #1066（右）处理，使用抗体为 TRAF1 (45D3) Rabbit mAb 兔单抗。 | |
Western blot analysis of extracts from Raji and SR cells, using TRAF1 (45D3) Rabbit mAb. Western blot 检测 Raji 和 SR 细胞提取物，使用抗体为TRAF1 (45D3) Rabbit mAb 兔单抗。 | |
Flow cytometric analysis of Raji cells, using TRAF1 (45D3) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red). 流式细胞仪检测Raji细胞，使用抗体为TRAF1 (45D3) Rabbit mAb 兔单抗（蓝色），用一个非特异性阴性对照抗体（红色）作为对照。 |
