| 货号 | 8025T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human,Monkey, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP |
| 目标/特异性 | SQSTM1/p62 (D5E2) Rabbit mAb recognizes endogenous levels of total SQSTM1/p62 protein. |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Sequestosome 1 (SQSTM1, p62) is a ubiquitin binding protein involved in cell signaling, oxidative stress, and autophagy (1-4). It was first identified as a protein that binds to the SH2 domain of p56Lck (5), and independently found to interact with PKCζ (6,7). It was subsequently found to interact with ubiquitin, providing a scaffold for several signaling proteins and triggering degradation of proteins through the proteasome or lysosome (8). Interaction between SQSTM1 and TRAF6 leads to the K63-linked polyubiquitination of TRAF6 and subsequent activation of the NF-κB pathway (9). Protein aggregates formed by SQSTM1 can be degraded by the autophagosome (4,10,11). SQSTM1 binds autophagosomal membrane protein LC3/Atg8, bringing SQSTM1-containing protein aggregates to the autophagosome (12). Lysosomal degradation of autophagosomes leads to a decrease in SQSTM1 levels during autophagy; conversely, autophagy inhibitors stabilize SQSTM1 levels. Studies have demonstrated a link between SQSTM1 and oxidative stress. SQSTM1 interacts with KEAP1, which is a cytoplasmic inhibitor of NRF2, a key transcription factor involved in cellular responses to oxidative stress (3). Thus, accumulation of SQSTM1 can lead to an increase in NRF2 activity. |
| 存放说明 | -20C |
| 计算分子量 | 62 |
| 参考文献 | 1 . Kirkin, V. et al. (2009) Mol Cell 34, 259-69. 2 . Seibenhener, M.L. et al. (2007) FEBS Lett 581, 175-9. 3 . Komatsu, M. et al. (2010) Nat Cell Biol 12, 213-23. 4 . Bjørkøy, G. et al. (2006) Autophagy 2, 138-9. 5 . Joung, I. et al. (1996) Proc Natl Acad Sci USA 93, 5991-5. 6 . Sanchez, P. et al. (1998) Mol Cell Biol 18, 3069-80. 7 . Puls, A. et al. (1997) Proc Natl Acad Sci USA 94, 6191-6. 8 . Vadlamudi, R.K. et al. (1996) J Biol Chem 271, 20235-7. 9 . Wooten, M.W. et al. (2005) J Biol Chem 280, 35625-9. 10 . Bjørkøy, G. et al. (2005) J Cell Biol 171, 603-14. 11 . Komatsu, M. et al. (2007) Cell 131, 1149-63. 12 . Pankiv, S. et al. (2007) J Biol Chem 282, 24131-45. |
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® SQSTM1/p62 siRNA I #6394 (+) or SignalSilence® SQSTM1/p62 siRNA II #6399 (+), using SQSTM1/p62 (D5E2) Rabbit mAb (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The SQSTM1/p62 (D5E2) Rabbit mAb confirms silencing of SQSTM1/p62 expression, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control. Western blot 分析HeLa细胞的细胞提取物，转染00 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-)，SignalSilence® SQSTM1/p62 siRNA I #6394 (+) 或 SignalSilence® SQSTM1/p62 siRNA II #6399 (+)，使用抗体是SQSTM1/p62 (D5E2) Rabbit mAb 兔单抗 (上图) 或 α-Tubulin (11H10) Rabbit mAb 兔单抗#2125 (下图)。SQSTM1/p62 (D5E2) Rabbit mAb 兔单抗证实SQSTM1/p62表达的沉默，α-Tubulin (11H10) Rabbit mAb 兔单抗用来检测内参。 | |
Western blot analysis of extracts from SK-MEL-2 cells, untreated (-) or starved overnight in Earles Balanced Salt Solution (EBSS) (+), using SQSTM1/p62 (D5E2) Rabbit mAb. Western blot 分析SK-MEL-2细胞的细胞提取物，未处理（-）或 Earles 平衡盐缓冲液（EBSS）饥饿过夜处理（+），使用抗体是SQSTM1/p62 (D5E2) Rabbit mAb 兔单抗。 | |
Western blot analysis of extracts from various cell lines using SQSTM1/p62 (D5E2) Rabbit mAb. Western blot分析多种细胞系的细胞提取物，使用抗体是SQSTM1/p62 (D5E2) Rabbit mAb 兔单抗。 | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a tagged human SQSTM1/p62 construct (+), using SQSTM1/p62 (D5E2) Rabbit mAb. Western blot分析293T细胞的细胞提取物，对照转染(-)或转染含有人源 SQSTM1/p62 的质粒(+)，使用抗体SQSTM1/p62 (D5E2) Rabbit mAb 兔单抗。 |
