TRA-1-60(S) (TRA-1-60(S)) Mouse mAb【科瑞奥博】

产品中心

当前位置：首页>产品中心

TRA-1-60(S) (TRA-1-60(S)) Mouse mAb

货号： 4746T 基本售价： 1350.0 元规格： -

产品信息

概述
货号	4746T
同种亚型	Mouse IgM
反应种属	Human,
来源宿主	Mouse IgM
应用	WB, IHC-P , F , IF-IC
目标/特异性	TRA-1-60(S) (TRA-1-60(S)) Mouse mAb detects endogenous levels of the neuraminidase-sensitive TRA-1-60 antigen.
使用方法	WB(1:1000) IHC-P (1:100) F (1:400) IF-IC (1:1000)

性能
供应商	CST
灵敏度	Endogenous
背景	TRA-1-60 and TRA-1-81 antibodies detect antigens present on the surface of human stem, teratocarcinoma, and embryonic germ cells (1). TRA-1-60(S) reacts with a neuraminidase sensitive epitope of a proteoglycan (2,3), while TRA-1-81 reacts with a neuraminidase insensitive epitope on the same antigen. Recently this antigen has been proposed to be a form of the protein podocalyxin (4). TRA-1-60 is also detected in the serum of patients with germ cell tumors (5,6).
存放说明	-20C
计算分子量	200-400
参考文献	1 . Andrews, P.W. et al. (1987) Int J Androl 10, 95-104. 2 . Andrews, P.W. et al. (1991) Recent Results Cancer Res 123, 63-83. 3 . Badcock, G. et al. (1999) Cancer Res 59, 4715-9. 4 . Schopperle, W.M. and DeWolf, W.C. (2007) Stem Cells 25, 723-30. 5 . Thomson, J.A. et al. (1998) Science 282, 1145-7. 6 . Marrink, J. et al. (1991) Int J Cancer 49, 368-72.

参考图片

Immunohistochemical analysis of paraffin-embedded NTERA (positive, left) and Jurkat (negative, right) cell pellets using TRA-1-60(S) (TRA-1-60(S)) Mouse mAb.使用TRA-1-60(S) (TRA-1-60(S))鼠源单克隆抗体对石蜡包埋的NTERA（阳性，左）和Jurkat（阴性，右）进行免疫组化分析。

Western blot analysis of extracts from various cell lines using TRA-1-60 (TRA-1-60) Mouse mAb.使用TRA-1-60（TRA-1-60）鼠源单克隆抗体对多种细胞提取物进行Western blot实验分析。

Flow cytometric analysis of unpermeabilized Jurkat cells (blue) and unpermeabilized NTERA2 cells (green) using TRA-1-60(S) (TRA-1-60(S)) Mouse mAb.使用TRA-1-60(S) (TRA-1-60(S))鼠源单克隆抗体对不可透化的Jurkat 细胞（蓝色）和不可透化的NTERA2细胞（绿色）进行流式细胞分析。

Confocal immunofluorescent analysis of nTERA2 cells using TRA-1-60(S) (TRA-1-60(S)) Mouse mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).使用TRA-1-60(S) (TRA-1-60(S)) 鼠源单克隆抗体（绿色）对nTERA2细胞进行激光共聚焦荧光分析实验。使用DY-554鬼笔环肽（红色）标记肌动蛋白丝。蓝色假色=DRAQ5® #4084 (荧光DNA染料).