| 货号 | 14475T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP , F , IF-IC |
| 目标/特异性 | Aurora A (D3E4Q) Rabbit mAb recognizes endogenous levels of total Aurora A protein. |
| 使用方法 | WB(1:1000) IP (1:50) F (1:50) IF-IC (1:200) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Aurora A (AIK) is a cell cycle-regulated Ser/Thr protein kinase that is overexpressed in many tumor cell lines (1-3). Phosphorylation of Aurora A at Thr288 within the kinase activation loop results in a significant increase in its activity and may target the protein for proteasomal degradation during mitosis (4). The closely-related kinase Aurora B (AIM1) has been implicated in multiple mitotic events (5), and siRNA silencing of Aurora B expression results in reduced histone H3 phosphorylation, aberrant chromosome alignment/segregation, and altered survivin localization (6). |
| 存放说明 | -20C |
| 计算分子量 | 48 |
| 参考文献 | 1 . Bischoff, J.R. et al. (1998) EMBO J 17, 3052-65. 2 . Zhou, H. et al. (1998) Nat Genet 20, 189-93. 3 . Sen, S. et al. (1997) Oncogene 14, 2195-200. 4 . Walter, A.O. et al. (2000) Oncogene 19, 4906-16. 5 . Kallio, M.J. et al. (2002) Curr Biol 12, 900-5. 6 . Hauf, S. et al. (2003) J Cell Biol 161, 281-94. |
Western blot analysis of extracts from HT-29 cells, untreated (-) or synchronized in mitosis by treatment with thymidine (2 mM, 17 hr; +) and Nocodazole #2190 (100 ng/ml, 24 hr; +), using Aurora A (D3E4Q) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Confocal immunofluorescent analysis of HeLa cells using Aurora A (D3E4Q) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Flow cytometric analysis of Jurkat cells using Aurora A (D3E4Q) Rabbit mAb and Propidium Iodide (PI)/RNase Staining Solution #4087 to measure DNA content. Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. | |
Immunoprecipitation of Aurora A from HeLa cell extracts using Rabbit (Da1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Aurora A (D3E4Q) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Aurora A (D3E4Q) Rabbit mAb. |
