| 货号 | 5128T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human,Mouse,Rat,Monkey, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB |
| 目标/特异性 | TCTP (D10F2) Rabbit mAb recognizes endogenous levels of total TCTP protein. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Translationally controlled tumor protein (TCTP/p23/HRF) is a ubiquitously expressed and highly conserved protein involved in various cellular processes, such as its role as a histamine releasing factor in chronic allergic disease (1). TCTP binds tubulin in a cell cycle dependent manner and is associated with the mitotic spindle (2). In addition, TCTP interacts with the actin cytoskeleton to regulate cell shape (3). In mitosis, TCTP is phosphorylated by PLK at Ser46, decreasing microtubule stability (4,5). TCTP interacts with the small GTPase Rheb, possibly acting as a GEF, thereby activating the TORC1 pathway and controlling cell growth and proliferation (6,7). TCTP has also been shown to be involved in apoptosis and cell stress (8-11). In cultured cells, reduction in TCTP expression can cause loss of the malignant phenotype (12).翻译控制的肿瘤蛋白（TCTP/p23/HRF）是一种广泛表达的高度保守的蛋白质，参与各种细胞过程，如它在慢性过敏性疾病可作为组胺释放因子（1）。TCTP以细胞周期依赖性的方式结合微管蛋白，并与有丝分裂纺锤体相关联（2）。此外，TCTP与肌动蛋白细胞骨架相互作用来调节细胞的形状（3）。在有丝分裂中，TCTP可被PLK磷酸化Ser46，降低微管稳定性（4,5）。 TCTP与小GTP酶Rheb相互作用，可能作为一个GEF，从而激活TORC1途径和控制细胞的生长和增殖（6,7）。TCTP也被证明参与了细胞凋亡和细胞应激（8-11）。在培养的细胞中，TCTP表达减少可能会导致恶性表型的缺失（12）。 |
| 存放说明 | -20C |
| 计算分子量 | 23 |
| 参考文献 | 1 . MacDonald, S.M. et al. (1995) Science 269, 688-90. 2 . Gachet, Y. et al. (1999) J Cell Sci 112 ( Pt 8), 1257-71. 3 . Bazile, F. et al. (2009) Carcinogenesis 30, 555-65. 4 . Yarm, F.R. (2002) Mol Cell Biol 22, 6209-21. 5 . Dephoure, N. et al. (2008) Proc Natl Acad Sci U S A 105, 10762-7. 6 . Dong, X. et al. (2009) J Biol Chem 284, 23754-64. 7 . Hsu, Y.C. et al. (2007) Nature 445, 785-8. 8 . Susini, L. et al. (2008) Cell Death Differ 15, 1211-20. 9 . Bommer, U.A. et al. (2009) Oncogene [Epub ahead of print]. 10 . Gnanasekar, M. et al. (2009) Biochem Biophys Res Commun 386, 333-7. 11 . Gnanasekar, M. et al. (2009) Int J Oncol 34, 1241-6. 12 . Tuynder, M. et al. (2002) Proc Natl Acad Sci USA 99, 14976-81. |
Western blot analysis of extracts from various cell lines using TCTP (D10F2) Rabbit mAb.Western blot方法检测不同细胞提取物，使用的抗体为TCTP (D10F2) Rabbit mAb。 |
