| 货号 | 4980T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human,Mouse,Rat,Monkey,Pig, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB |
| 目标/特异性 | Csk (C74C1) Rabbit mAb detects endogenous levels of total Csk protein. This antibody does not cross-react with other proteins. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Carboxy-terminal Src kinase (Csk) is a ubiquitously expressed nonreceptor tyrosine kinase that negatively regulates the Src family kinases (SFKs) by phosphorylation of the SFK carboxy-terminal tyrosine (1,2). Phosphorylated carboxy-terminal tyrosine binds to the SH2 domain of SFK intramolecularly and leads to folding and inactivation of the SFK (3). This Csk-catalyzed SFK tyrosine phosphorylation is highly specific and exclusive. The SFK carboxy-terminal tyrosine is the only known physiological substrate of Csk (4). Csk consists of an SH2, an SH3, and a kinase domain. There is evidence that the SH2 and SH3 domains are essential for the regulation of SFK, and Csk can be recruited to the membrane where SFKs are in an active state. This process is mediated by a Csk-binding protein (Cbp, also called PAG), which binds tightly to the SH2 domain of Csk (5). Activation of SFK by extracellular stimuli leads to the tyrosine phosphorylation of Cbp, generating docking sites for Csk. The recruitment of Csk forms a feedback mechanism for termination of SFK function (6). |
| 存放说明 | -20C |
| 计算分子量 | 50 |
| 参考文献 | 1 . Nada, S. et al. (1991) Nature 351, 69-72. 2 . Nada, S. et al. (1993) Cell 73, 1125-35. 3 . Lee, S. et al. (2003) Proc Natl Acad Sci U S A 100, 14707-12. 4 . Imamoto, A. and Soriano, P. (1993) Cell 73, 1117-24. 5 . Kawabuchi, M. et al. (2000) Nature 404, 999-1003. 6 . Matsuoka, H. et al. (2004) J Biol Chem 279, 5975-83. |
Western blot analysis of extracts from various cell lines using Csk (C74C1) Rabbit mAb.Western blot方法检测细胞提取物：各种细胞系，使用的抗体是Csk (C74C1) Rabbit mAb。 |
