| 货号 | 15035T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human,Mouse,Rat, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IF-F |
| 目标/特异性 | Neurofascin 155 (D7B6O) recognizes endogenous levels of total neurofascin 155 protein. |
| 使用方法 | WB(1:1000) IF-F (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Myelinated axons contain un-myelinated gaps called nodes of Ranvier. These regularly spaced gaps are critical for the proper propagation and rapid conduction of nerve impulses in the central and peripheral nervous system (1). The structure and organization of the nodes of Ranvier is dictated by interaction between the axon and glial cells (2). Voltage-gated sodium channels concentrated at the nodes and potassium channels clustered at the paranodes are responsible for propagation of the action potentials (3,4). Other proteins that contribute to the architecture and function of the nodes of Ranvier include βIV spectrin (5), ankyrin-G (6), and the L1 cell adhesion molecules, neurofascin and NrCAM (7,8). |
| 存放说明 | -20C |
| 计算分子量 | 140-155 |
| 参考文献 | 1 . Black, J.A. et al. (1990) Trends Neurosci 13, 48-54. 2 . Salzer, J.L. (1997) Neuron 18, 843-6. 3 . Waxman, S.G. et al. (1989) Proc Natl Acad Sci U S A 86, 1406-10. 4 . Ritchie, J.M. (1992) Trends Neurosci 15, 345-51. 5 . Berghs, S. et al. (2000) J Cell Biol 151, 985-1002. 6 . Zhou, D. et al. (1998) J Cell Biol 143, 1295-304. 7 . Davis, J.Q. et al. (1996) J Cell Biol 135, 1355-67. 8 . Ratcliffe, C.F. et al. (2001) J Cell Biol 154, 427-34. 9 . Thaxton, C. et al. (2011) Neuron 69, 244-57. 10 . Charles, P. et al. (2002) Curr Biol 12, 217-20. 11 . Pomicter, A.D. et al. (2010) Brain 133, 389-405. |
Confocal immunofluorescent analysis of rat spinal cord using Neurofascin 186 (D6G6O) Rabbit mAb (left, green) or Neurofascin 155 (D7B6O) Rabbit mAb # 15035 (right, green). β3-Tubulin protein was labeled with β3-Tubulin (TU-20) Mouse mAb #4466 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Western blot analysis of extracts from mouse brain, mouse lung, and rat brain using Neurofascin 155 (D7B6O) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Confocal immunofluorescent analysis of mouse sciatic nerve using Neurofascin 186 (D6G6O) Rabbit mAb (left, green) or Neurofascin 155 (D7B6O) Rabbit mAb # 15035 (right, green). Neurofilament-L protein was labeled with Neurofilament-L (DA2) Mouse mAb #2835 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). |
