| 货号 | 15071T |
| 同种亚型 | Mouse IgG1 |
| 反应种属 | Human, |
| 来源宿主 | Mouse IgG1 |
| 应用 | WB, IP , IHC-P , F |
| 目标/特异性 | Bcl-2 (124) Mouse mAb recognizes endogenous levels of total Bcl-2 protein. |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:400) F (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Bcl-2 exerts a survival function in response to a wide range of apoptotic stimuli through inhibition of mitochondrial cytochrome c release (1). It has been implicated in modulating mitochondrial calcium homeostasis and proton flux (2). Several phosphorylation sites have been identified within Bcl-2 including Thr56, Ser70, Thr74, and Ser87 (3). It has been suggested that these phosphorylation sites may be targets of the ASK1/MKK7/JNK1 pathway and that phosphorylation of Bcl-2 may be a marker for mitotic events (4,5). Mutation of Bcl-2 at Thr56 or Ser87 inhibits its anti-apoptotic activity during glucocorticoid-induced apoptosis of T lymphocytes (6). Interleukin-3 and JNK-induced Bcl-2 phosphorylation at Ser70 may be required for its enhanced anti-apoptotic functions (7). |
| 存放说明 | -20C |
| 计算分子量 | 26 |
| 参考文献 | 1 . Murphy, K.M. et al. (2000) Cell Death Differ 7, 102-11. 2 . Zhu, L. et al. (1999) J Biol Chem 274, 33267-73. 3 . Maundrell, K. et al. (1997) J Biol Chem 272, 25238-42. 4 . Yamamoto, K. et al. (1999) Mol Cell Biol 19, 8469-78. 5 . Ling, Y.H. et al. (1998) J Biol Chem 273, 18984-91. 6 . Huang, S.T. and Cidlowski, J.A. (2002) FASEB J 16, 825-32. 7 . Deng, X. et al. (2001) J Biol Chem 276, 23681-8. |
Immunohistochemical analysis of paraffin-embedded RL-7 (positive, left) and HT-29 (negative, right) cell pellets using Bcl-2 (124) Mouse mAb. | |
Immunohistochemical analysis of paraffin-embedded human tonsil using Bcl-2 (124) Mouse mAb. | |
Immunoprecipitation of Bcl-2 from RL-7 cell extracts using Mouse (G3A1) mAb IgG1 Isotype Control #5415 (lane 2) or Bcl-2 (124) Mouse mAb (lane 3). Lane 1 is 10% input. Western blot was performed using Bcl-2 (D55G8) Rabbit mAb (Human Specific) #4223. | |
Western blot analysis of extracts from various cell lines using Bcl-2 (124) Mouse mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). KARPAS cell Line source: Dr Abraham Karpas at the University of Cambridge. | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Bcl-2 (124) Mouse mAb. | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Bcl-2 (124) Mouse mAb. | |
Flow cytometric analysis of HT29 cells (blue), SHSY5Y cells (red) and RL7 cells (green) using Bcl-2 Mouse mAb. Anti-mouse IgG (H+L), F(ab)2 fragment (Alexa Fluor 488 Conjugate) #4412 was used as a secondary antibody. |
