| 货号 | 5276T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP |
| 目标/特异性 | Notch3 (D11B8) Rabbit mAb detects endogenous levels of total Notch3 protein. The antibody recognizes both full-length (FL) Notch3 at 270 kDa and a truncated protein (NTM) containing a short extracellular region, the transmembrane domain and the intracellular region at 90 kDa. |
| 使用方法 | WB(1:1000) IP (1:200) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Notch1 is a transmembrane protein functioning in development and the determination of cell fate (1). During maturation, the notch molecule is cleaved by a furin-like convertase at its extracellular domain (2). Upon binding to a ligand such as Delta1, or upon extracellular calcium depletion, the carboxy-terminal notch1 fragment is released and further cleaved between Gly1743 and Val1744 (3,4). The resulting activated cytosolic fragment translocates to the nucleus where it activates transcription. |
| 存放说明 | -20C |
| 计算分子量 | 90, 270 |
| 参考文献 | 1 . Artavanis-Tsakonas, S. et al. (1999) Science 284, 770-6. 2 . Chan, Y.M. and Jan, Y.N. (1998) Cell 94, 423-6. 3 . Schroeter, E.H. et al. (1998) Nature 393, 382-6. 4 . Rand, M.D. et al. (2000) Mol Cell Biol 20, 1825-35. 5 . Baron, M. (2003) Semin Cell Dev Biol 14, 113-9. 6 . Kalimo, H. et al. (2002) Brain Pathol 12, 371-84. 7 . Karlström, H. et al. (2002) Proc Natl Acad Sci U S A 99, 17119-24. 8 . Monet, M. et al. (2007) Hum Mol Genet 16, 982-92. 9 . Park, J.T. et al. (2006) Cancer Res 66, 6312-8. 10 . Gramantieri, L. et al. (2007) Liver Int 27, 997-1007. 11 . Yamaguchi, N. et al. (2008) Cancer Res 68, 1881-8. |
Western blot analysis of extracts from various cell lines using Notch3 (D11B8) Rabbit mAb. 使用Notch3（D11B8）Rabbit mAb兔单抗对多种细胞提取物进行Western blot分析。 |
