| 货号 | 5306T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human,Mouse,Rat, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB |
| 目标/特异性 | AMPA Receptor (GluR 2) (D39F2) Rabbit mAb detects endogenous levels of total GluR 2 protein. The antibody is not predicted to recognize other AMPA receptor subunits (e.g. GluR 1, GluR 3 or GluR 4) based on sequence homology of the antigen. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | AMPA- (α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid), kainite- and NMDA- (N-methyl-D-aspartate) receptors are the three main families of ionotropic glutamate-gated ion channels. AMPA receptors (AMPARs) are comprised of four subunits (GluR 1-4), which assemble as homo- or hetero-tetramers to mediate the majority of fast excitatory transmissions in the CNS. AMPARs are implicated in synapse formation, stabilization, and plasticity (1). AMPARs that lack GluR 2 are permeable to calcium, in contrast to GluR 2 containing AMPARs (2). Post-transcriptional modifications (alternative splicing, nuclear RNA editing) and post-translational modifications (glycosylation, phosphorylation) result in a very large number of permutations, fine-tuning the kinetic properties of AMPARs. Activity changes of AMPARs are implicated in a variety of diseases including Alzheimer’s, amyotrophic lateral sclerosis (ALS), stroke, and epilepsy (1).Src family tyrosine kinases phosphorylate the GluR 2 subunit of AMPA receptors at Tyr876, which increases the interaction with GRIP1/2 but not PICK1. In addition, Tyr876 is important for AMPA- and NMDA-induced GluR 2 internalization (3). The phosphorylation sites at Tyr869, Tyr873 and Tyr876 were identified at Cell Signaling Technology (CST) using PhosphoScan®, CSTs MS/MS platform for phosphorylation site discovery (4). Phosphorylation of GluR 2 at Tyr869, Tyr873 and Tyr876 was observed in extracts isolated from ischemic rat brain. These sites were independently found in a large-scale identification of tyrosine phosphorylation sites from murine brain (5). |
| 存放说明 | -20C |
| 计算分子量 | 100 |
| 参考文献 | 1 . Palmer, C.L. et al. (2005) Pharmacol Rev 57, 253-77. 2 . Cull-Candy, S. et al. (2006) Curr Opin Neurobiol 16, 288-97. 3 . Hayashi, T. and Huganir, R.L. (2004) J Neurosci 24, 6152-60. 4 . Ballif, B.A. et al. (2008) J Proteome Res 7, 311-8. 5 . Rush, J. et al. (2005) Nat Biotechnol 23, 94-101. |
Western blot analysis of extracts from mouse and rat brains using AMPA Receptor (GluR 2) (D39F2) Rabbit mAb. Western blot分析小鼠和大鼠大脑提取物，使用的抗体是AMPA Receptor (GluR 2) (D39F2) Rabbit mAb 兔单抗。 |
