MDA-5 (D74E4) Rabbit mAb【科瑞奥博】

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MDA-5 (D74E4) Rabbit mAb

货号： 5321T 基本售价： 1350.0 元规格： -

产品信息

概述
货号	5321T
同种亚型	Rabbit IgG
反应种属	Human,Mouse,
来源宿主	Rabbit IgG
应用	WB, IP
目标/特异性	MDA-5 (D74E4) Rabbit mAb detects endogenous levels of total MDA-5 protein.
使用方法	WB(1:1000) IP (1:100)

性能
供应商	CST
灵敏度	Endogenous
背景	Antiviral innate immunity depends on the combination of parallel pathways triggered by virus detecting proteins in the Toll-like receptor (TLR) family and RNA helicases, such as Rig-I (retinoic acid-inducible gene I) and MDA-5 (melanoma differentiation-associated antigen 5), which promote the transcription of type I interferons (IFN) and antiviral enzymes (1-3). TLRs and helicase proteins contain sites that recognize the molecular patterns of different virus types, including DNA, single-stranded RNA (ssRNA), double-stranded RNA (dsRNA), and glycoproteins. These antiviral proteins are found in different cell compartments; TLRs (i.e. TLR3, TLR7, TLR8, and TLR9) are expressed on endosomal membranes and helicases are localized to the cytoplasm. Rig-I expression is induced by retinoic acid, LPS, IFN, and viral infection (4,5). Both Rig-I and MDA-5 share a DExD/H-box helicase domain that detects viral dsRNA and two amino-terminal caspase recruitment domains (CARD) that are required for triggering downstream signaling (4-7). Rig-I binds both dsRNA and viral ssRNA that contains a 5-triphosphate end not seen in host RNA (8,9). Though structurally related, Rig-I and MDA-5 detect a distinct set of viruses (10,11). The CARD domain of the helicases, which is sufficient to generate signaling and IFN production, is recruited to the CARD domain of the MAVS/VISA/Cardif/IPS-1 mitochondrial protein, which triggers activation of NF-κB, TBK1/IKKε, and IRF-3/IRF-7 (12-15).
存放说明	-20C
计算分子量	135
参考文献	1 . Yoneyama, M. and Fujita, T. (2007) J Biol Chem 282, 15315-8. 2 . Meylan, E. and Tschopp, J. (2006) Mol Cell 22, 561-9. 3 . Thompson, A.J. and Locarnini, S.A. (2007) Immunol Cell Biol 85, 435-45. 4 . Imaizumi, T. et al. (2002) Biochem Biophys Res Commun 292, 274-9. 5 . Zhang, X. et al. (2000) Microb Pathog 28, 267-78. 6 . Yoneyama, M. et al. (2005) J Immunol 175, 2851-8. 7 . Yoneyama, M. et al. (2004) Nat. Immunol. 5, 730-737. 8 . Hornung, V. et al. (2006) Science 314, 994-7. 9 . Pichlmair, A. et al. (2006) Science 314, 997-1001. 10 . Kato, H. et al. (2006) Nature 441, 101-5. 11 . Childs, K. et al. (2007) Virology 359, 190-200. 12 . Meylan, E. et al. (2005) Nature 437, 1167-72. 13 . Xu, L.G. et al. (2005) Mol. Cell 19, 727-740. 14 . Kawai, T. et al. (2005) Nat Immunol 6, 981-8. 15 . Seth, R.B. et al. (2005) Cell 122, 669-682. 16 . Kato, H. et al. (2006) Nature 441, 101-5. 17 . Kang, D.C. et al. (2002) Proc Natl Acad Sci U S A 99, 637-42. 18 . Kang, D.C. et al. (2004) Oncogene 23, 1789-800. 19 . Cocude, C. et al. (2003) J Gen Virol 84, 3215-25. 20 . Kovacsovics, M. et al. (2002) Curr Biol 12, 838-43. 21 . Berghäll, H. et al. (2006) Microbes Infect 8, 2138-44.

参考图片

Western blot anlaysis of extracts from differentiated THP-1 cells, untreated or treated with LPS (1 μg/ml, indicated times), using MDA-5 (D74E4) Rabbit mAb.Western免疫印迹。用MDA-5 (D74E4) Rabbit mAb研究未经处理的和经 LPS (1 μg/ml)处理一定时间分化了的THP-1细胞的细胞提取液。

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with human MDA-5 (+), using MDA-5 (D74E4) Rabbit mAb.Western免疫印迹。用 MDA-5 (D74E4) Rabbit mAb研究对照转染(-)和转染了人MDA-5 (+)的293T细胞的细胞提取液。