| 货号 | 13331T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human,Monkey, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP |
| 目标/特异性 | CDK6 (D4S8S) Rabbit mAb recognizes endogenous levels of total CDK6 protein. This antibody does not cross-react with other CDK proteins. |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The cyclin-dependent kinases form complexes with their cyclin partners and with CDK inhibitors. CDK6 and CDK4 associate with the D-type cyclins and target the retinoblastoma protein, allowing passage through the G1/S phase restriction point (1). CDK6/cyclin D complexes are sequestered in their inactive form through binding to one of the INK4 CDK inhibitor family members (2,3). Unlike the INK4 family of CDK inhibitors, the CDK inhibitor p21 Waf1/Cip1 may enhance the association of CDK4 and CDK6 with their cyclin D partners (4). |
| 存放说明 | -20C |
| 计算分子量 | 36 |
| 参考文献 | 1 . Lukas, J. et al. (1996) Mol Cell Biol 16, 6917-25. 2 . Hirai, H. et al. (1995) Mol Cell Biol 15, 2672-81. 3 . Pestell, R.G. et al. (1999) Endocr Rev 20, 501-34. 4 . Flores-Rozas, H. et al. (1994) Proc Natl Acad Sci U S A 91, 8655-9. |
Western blot analysis of extracts from HeLa, Jurkat, and COS-7 cells using CDK6 (D4S8S) Rabbit mAb. 使用CDK6 (D4S8S) Rabbit mAb对HeLa，Jurkat 和COS-7细胞提取物进行western blot分析。 | |
Immunoprecipitation of CDK6 from HeLa cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or CDK6 (D4S8S) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot was performed using CDK6 (DCS83) Mouse mAb #3136.使用Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) 或 CDK6 (D4S8S) Rabbit mAb (lane 3)对HeLa细胞提取物的CDK6进行免疫沉淀实验。Lane1是10%上样量。使用CDK6 (DCS83) Mouse mAb #3136进行western blot分析。 |
