| 货号 | 5362T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP |
| 目标/特异性 | Dicer (D38E7) Rabbit mAb detects endogenous levels of total Dicer protein. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Dicer is a member of the RNase III family that specifically cleaves double-stranded RNAs to generate microRNAs (miRNAs) (1). After long primary transcript pri-miRNAs are processed to stem-looped pre-miRNAs by Drosha (2), pre-miRNAs are transported to the cytoplasm and further processed by Dicer to produce 22-nucleotide mature miRNAs (3). The mature miRNA then becomes a part of the RNA-Induced Silencing Complex (RISC) and can bind to the 3 UTR of the target mRNA (3). |
| 存放说明 | -20C |
| 计算分子量 | 220 |
| 参考文献 | 1 . Hutvágner, G. and Zamore, P.D. (2002) Science 297, 2056-60. 2 . Lee, Y. et al. (2003) Nature 425, 415-9. 3 . Diederichs, S. and Haber, D.A. (2007) Cell 131, 1097-108. |
Western blot analysis of extracts from MCF7 and 293 cells using Dicer (D38E7) Rabbit mAb. 使用Dicer (D38E7) Rabbit mAb，免疫印迹（Western Blotting）分析MCF7和293细胞系裂解物。 |
