| 货号 | 5377T |
| 同种亚型 | Mouse IgG1 |
| 反应种属 | Human,Mouse,Rat,Monkey, |
| 来源宿主 | Mouse IgG1 |
| 应用 | WB, IP , IF-IC |
| 目标/特异性 | JMJD1B (6A1-1F5) Mouse mAb detects endogenous levels of total JMJD1B protein. |
| 使用方法 | WB(1:1000) IP (1:50) IF-IC (1:200) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The methylation state of lysine residues in histone proteins is a major determinant of the formation of active and inactive regions of the genome and is crucial for the proper programming of the genome during development (1,2). Jumonji C (JmjC) domain-containing proteins represent the largest class of potential histone demethylase proteins (3). The JmjC domain of several proteins has been shown to catalyze the demethylation of mono-, di-, and tri-methyl lysine residues via an oxidative reaction that requires iron and α-ketoglutarate (3). Based on homology, both humans and mice contain at least 30 such proteins, which can be divided into seven separate families (3). The JMJD1 (Jumonji domain-containing protein 1) family, also known as JHDM2 (JmjC domain-containing histone demethylation protein 2) family, contains four members: hairless (HR), JMJD1A/JHDM2A, JMJD1B/JHDM2B, and JMJD1C/JHDM2C. Hairless is expressed in the skin and brain and acts as a co-repressor of the thyroid hormone receptor (4-6). Mutations in the hairless gene cause alopecia in both mice and humans (4,5). JMJD1A is expressed in meiotic and post-meiotic male germ cells, contributes to androgen receptor-mediated gene regulation, and is required for spermatogenesis (7-9). It has also been identified as a downstream target of OCT4 and STAT3 and is critical for the regulation of self-renewal in embryonic stem cells (10,11). JMJD1B is a more widely expressed family member and is frequently deleted in myeloid leukemia (12). JMJD1C (also known as TRIP8) is a co-factor of both the androgen and thyroid receptors and has a potential link to autism (13-15). Members of the JMJD1/JHDM2 family have been shown to demethylate mono-methyl and di-methyl histone H3 (Lys9) (3,8). |
| 存放说明 | -20C |
| 计算分子量 | 220 |
| 参考文献 | 1 . Kubicek, S. et al. (2006) Ernst Schering Res Found Workshop , 1-27. 2 . Lin, W. and Dent, S.Y. (2006) Curr Opin Genet Dev 16, 137-42. 3 . Klose, R.J. et al. (2006) Nat Rev Genet 7, 715-27. 4 . Cachon-Gonzalez, M.B. et al. (1994) Proc Natl Acad Sci USA 91, 7717-21. 5 . Ahmad, W. et al. (1998) Science 279, 720-4. 6 . Potter, G.B. et al. (2001) Genes Dev 15, 2687-701. 7 . Höög, C. et al. (1991) Mol Reprod Dev 30, 173-81. 8 . Yamane, K. et al. (2006) Cell 125, 483-95. 9 . Okada, Y. et al. (2007) Nature 450, 119-23. 10 . Loh, Y.H. et al. (2007) Genes Dev 21, 2545-57. 11 . Ko, S.Y. et al. (2006) Cell Struct Funct 31, 53-62. 12 . Hu, Z. et al. (2001) Oncogene 20, 6946-54. 13 . Lee, J.W. et al. (1995) Mol Endocrinol 9, 243-54. 14 . Wolf, S.S. et al. (2007) Arch Biochem Biophys 460, 56-66. 15 . Castermans, D. et al. (2007) Eur J Hum Genet 15, 422-31. |
Western blot analysis of extracts from NCCIT cells using JMJD1B (6A1-1F5) Mouse mAb. 使用JMJD1B (6A1-1F5) Mouse mAb，免疫印迹(Western blot)分析NCCIT细胞中JMJD1B (6A1-1F5)的蛋白水平。 | |
Confocal immunofluorescent analysis of HeLa cells using JMJD1B (6A1-1F5) Mouse mAb (green). Actin filaments were labeled using DY-554 phalloidin (red). 使用JMJD1B (6A1-1F5) Mouse mAb (绿色)标记，共聚焦免疫荧光分析HeLa细胞。DY-554 phalloidin标记微丝蛋白(红色)。 |
